Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30819 | 5' | -57 | NC_006552.1 | + | 19495 | 0.69 | 0.493169 |
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Target: 5'- uUCCGaacugCUCGCGCugCCGgAGCCa- -3' miRNA: 3'- -AGGUgca--GAGCGCGugGGUgUUGGcu -5' |
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| 30819 | 5' | -57 | NC_006552.1 | + | 43536 | 0.7 | 0.463583 |
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Target: 5'- cUCCuCGUCUCGCGCGgcguCCCgaACAGCUu- -3' miRNA: 3'- -AGGuGCAGAGCGCGU----GGG--UGUUGGcu -5' |
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| 30819 | 5' | -57 | NC_006552.1 | + | 14111 | 0.7 | 0.444384 |
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Target: 5'- cUUCAUGUCgCGCGCAUCCGgcGCCGu -3' miRNA: 3'- -AGGUGCAGaGCGCGUGGGUguUGGCu -5' |
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| 30819 | 5' | -57 | NC_006552.1 | + | 36394 | 0.71 | 0.372354 |
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Target: 5'- cUCCGCGUCUCGCcgGCGCUCGgAgacGCCu- -3' miRNA: 3'- -AGGUGCAGAGCG--CGUGGGUgU---UGGcu -5' |
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| 30819 | 5' | -57 | NC_006552.1 | + | 8551 | 0.75 | 0.228738 |
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Target: 5'- gUCCACG--UCGCGCGCCCACAucaacguGCCc- -3' miRNA: 3'- -AGGUGCagAGCGCGUGGGUGU-------UGGcu -5' |
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| 30819 | 5' | -57 | NC_006552.1 | + | 7012 | 0.79 | 0.131946 |
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Target: 5'- aCUGCGUCUCGCGCacgGCCCGgCGugCGAa -3' miRNA: 3'- aGGUGCAGAGCGCG---UGGGU-GUugGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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