Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30821 | 5' | -59.5 | NC_006552.1 | + | 27595 | 0.66 | 0.522804 |
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Target: 5'- --aGCGGGaaaggcuugaggAUGCCCAGGuagguGCUGAUGCg -3' miRNA: 3'- aagCGCCU------------UGUGGGUCCc----CGGCUACG- -5' |
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| 30821 | 5' | -59.5 | NC_006552.1 | + | 48741 | 0.66 | 0.522804 |
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Target: 5'- --aGUGGAAUcgcacgcacccgGCCCAGGuGGCCGuUGa -3' miRNA: 3'- aagCGCCUUG------------UGGGUCC-CCGGCuACg -5' |
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| 30821 | 5' | -59.5 | NC_006552.1 | + | 32825 | 0.66 | 0.49151 |
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Target: 5'- --gGCGGucggugaAGCuuuCCCGcGGGCCGAUGUa -3' miRNA: 3'- aagCGCC-------UUGu--GGGUcCCCGGCUACG- -5' |
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| 30821 | 5' | -59.5 | NC_006552.1 | + | 57255 | 0.67 | 0.443921 |
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Target: 5'- --aGCGGAACGCCCuc--GCCGAcgGCg -3' miRNA: 3'- aagCGCCUUGUGGGucccCGGCUa-CG- -5' |
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| 30821 | 5' | -59.5 | NC_006552.1 | + | 17356 | 0.68 | 0.398147 |
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Target: 5'- gUUCGCGGAGCGa-CGGGuugcGCCGAcUGCg -3' miRNA: 3'- -AAGCGCCUUGUggGUCCc---CGGCU-ACG- -5' |
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| 30821 | 5' | -59.5 | NC_006552.1 | + | 3016 | 0.68 | 0.39638 |
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Target: 5'- -gCGCGGAGCAgaucgugcuccaCCAGGGcgaGCCGA-GCg -3' miRNA: 3'- aaGCGCCUUGUg-----------GGUCCC---CGGCUaCG- -5' |
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| 30821 | 5' | -59.5 | NC_006552.1 | + | 21596 | 0.69 | 0.363779 |
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Target: 5'- -aUGCGGAugcaccagcGCGCCCu--GGCCGAUGUc -3' miRNA: 3'- aaGCGCCU---------UGUGGGuccCCGGCUACG- -5' |
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| 30821 | 5' | -59.5 | NC_006552.1 | + | 26684 | 0.7 | 0.311707 |
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Target: 5'- -aUGCGGAucgACcuuuccccacuggucGCCCAGGGcGCCGAagGCg -3' miRNA: 3'- aaGCGCCU---UG---------------UGGGUCCC-CGGCUa-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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