Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30823 | 3' | -57.2 | NC_006552.1 | + | 38091 | 0.69 | 0.462289 |
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Target: 5'- cGAGGAacGGCaGGACAGCAgccUGGUacugCGGCg -3' miRNA: 3'- -CUUCU--UCGgCCUGUCGU---GCCAg---GCCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 5596 | 0.69 | 0.452608 |
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Target: 5'- --cGAAGCCGGcCAgGCugACGGUCagcgCGGCa -3' miRNA: 3'- cuuCUUCGGCCuGU-CG--UGCCAG----GCCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 35386 | 0.7 | 0.42425 |
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Target: 5'- aGAGAcuGGCCGG-CGGUuCGGUCUuGGCu -3' miRNA: 3'- cUUCU--UCGGCCuGUCGuGCCAGG-CCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 41401 | 0.7 | 0.39698 |
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Target: 5'- cAGGGucAGCCaGGCAGCGCGccaGUUCGGCc -3' miRNA: 3'- cUUCU--UCGGcCUGUCGUGC---CAGGCCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 45367 | 0.71 | 0.354117 |
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Target: 5'- ----cGGCCGGuaucugGCAGCGCGGcCCGGg -3' miRNA: 3'- cuucuUCGGCC------UGUCGUGCCaGGCCg -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 59921 | 0.72 | 0.337911 |
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Target: 5'- cAAGGcgcGCCGGACcGUGCuGUCCGGCu -3' miRNA: 3'- cUUCUu--CGGCCUGuCGUGcCAGGCCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 61369 | 0.73 | 0.271751 |
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Target: 5'- -cGGGAGCCGGGCcauguuuuGGCGCG--CCGGCg -3' miRNA: 3'- cuUCUUCGGCCUG--------UCGUGCcaGGCCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 32699 | 0.74 | 0.227809 |
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Target: 5'- --cGucGCCGGcCGGCGCGGggCCGGUg -3' miRNA: 3'- cuuCuuCGGCCuGUCGUGCCa-GGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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