Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30827 | 5' | -53.1 | NC_006552.1 | + | 45289 | 0.66 | 0.87502 |
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Target: 5'- aUCGagUGcgaUAGGCCaUGGCGUAGUUGg -3' miRNA: 3'- cAGCa-AC---AUCUGGaGCCGCAUCGACg -5' |
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| 30827 | 5' | -53.1 | NC_006552.1 | + | 40305 | 0.66 | 0.858904 |
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Target: 5'- uUCGUUGUGuGCCUCGuaGUAGUc-- -3' miRNA: 3'- cAGCAACAUcUGGAGCcgCAUCGacg -5' |
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| 30827 | 5' | -53.1 | NC_006552.1 | + | 41005 | 0.67 | 0.810812 |
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Target: 5'- uGUUGUUGUGGGCgaacagcaguucaUCcGCGuUGGCUGCg -3' miRNA: 3'- -CAGCAACAUCUGg------------AGcCGC-AUCGACG- -5' |
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| 30827 | 5' | -53.1 | NC_006552.1 | + | 6362 | 0.67 | 0.805099 |
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Target: 5'- uGUCGagcaccUUGUAGcCCUUGGCGUccCUGUa -3' miRNA: 3'- -CAGC------AACAUCuGGAGCCGCAucGACG- -5' |
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| 30827 | 5' | -53.1 | NC_006552.1 | + | 15000 | 0.73 | 0.495496 |
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Target: 5'- uGUCGguggcgGUAGGCUUCGGCGccagUGGCUucaGCa -3' miRNA: 3'- -CAGCaa----CAUCUGGAGCCGC----AUCGA---CG- -5' |
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| 30827 | 5' | -53.1 | NC_006552.1 | + | 31983 | 0.74 | 0.406709 |
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Target: 5'- -----gGUGGugCUCGGCGUgaaucuccGGCUGCg -3' miRNA: 3'- cagcaaCAUCugGAGCCGCA--------UCGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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