miRNA: 3'- gcuucauCUACG-CCCGCG---UCCGAACc -5'
miRNA: 3'- -GCuuCA------------UCUa---CGCCCgcGUCCGAACC- -5'
miRNA: 3'- gCUUCAUCuaCGcCCGcGUCCGAACC- -5'
miRNA: 3'- gcuucauCUACGCCCG-CGUCCGaACC- -5'
miRNA: 3'- gcuucauCUACGC------CCGCGUCCGAACC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30829 | 5' | -54.8 | NC_006552.1 | + | 14842 | 0.66 | 0.775791 |
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Target: 5'- ------cGAUGCuGGGCGCcuuGGGCUUGu -3' miRNA: 3'- gcuucauCUACG-CCCGCG---UCCGAACc -5' |
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| 30829 | 5' | -54.8 | NC_006552.1 | + | 13271 | 0.66 | 0.755627 |
|
Target: 5'- uCGggGUuuucgaaucgcuGGGcugcGCGGGauCGGGCUUGGg -3' miRNA: 3'- -GCuuCA------------UCUa---CGCCCgcGUCCGAACC- -5' |
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| 30829 | 5' | -54.8 | NC_006552.1 | + | 57472 | 0.67 | 0.734968 |
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Target: 5'- gGAGGUGGc-GCcGGUaCGGGCUUGGa -3' miRNA: 3'- gCUUCAUCuaCGcCCGcGUCCGAACC- -5' |
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| 30829 | 5' | -54.8 | NC_006552.1 | + | 30082 | 0.69 | 0.616432 |
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Target: 5'- ------cGAUGCGGGUaGCAGGC-UGGc -3' miRNA: 3'- gcuucauCUACGCCCG-CGUCCGaACC- -5' |
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| 30829 | 5' | -54.8 | NC_006552.1 | + | 17594 | 0.69 | 0.583828 |
|
Target: 5'- ------cGAUGCGcugggcGGCGCGGGUUUGGa -3' miRNA: 3'- gcuucauCUACGC------CCGCGUCCGAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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