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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30831 | 5' | -56.6 | NC_006552.1 | + | 13329 | 0.66 | 0.680347 |
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Target: 5'- gGACuGGGGCCGGGcuGCauccUCGGcaCGCACg -3' miRNA: 3'- -CUG-CCCCGGCUUauCG----AGUCa-GCGUG- -5' |
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| 30831 | 5' | -56.6 | NC_006552.1 | + | 56127 | 0.66 | 0.66847 |
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Target: 5'- aGACGGGGCgaugaacgcgcaCGGAUcucgcgaGGCUCAGcCGUgaaGCa -3' miRNA: 3'- -CUGCCCCG------------GCUUA-------UCGAGUCaGCG---UG- -5' |
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| 30831 | 5' | -56.6 | NC_006552.1 | + | 22106 | 0.7 | 0.429428 |
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Target: 5'- uGACGGGuGCCGAAU-GCUUcGUCGgcCACc -3' miRNA: 3'- -CUGCCC-CGGCUUAuCGAGuCAGC--GUG- -5' |
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| 30831 | 5' | -56.6 | NC_006552.1 | + | 4812 | 0.71 | 0.361244 |
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Target: 5'- cGGCGGGGgCGAu--GCgcacgcgcacagggCGGUCGCGCa -3' miRNA: 3'- -CUGCCCCgGCUuauCGa-------------GUCAGCGUG- -5' |
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| 30831 | 5' | -56.6 | NC_006552.1 | + | 32712 | 0.72 | 0.309946 |
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Target: 5'- cGCGGGGCCGGuggagcGCUCgaacaGGUCGCGa -3' miRNA: 3'- cUGCCCCGGCUuau---CGAG-----UCAGCGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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