Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30832 | 5' | -53.9 | NC_006552.1 | + | 43006 | 0.66 | 0.858102 |
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Target: 5'- gGCGACCuUGGucuUCACCCGG--GCa-- -3' miRNA: 3'- -CGCUGGcAUCu--AGUGGGCCaaCGacu -5' |
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| 30832 | 5' | -53.9 | NC_006552.1 | + | 33422 | 0.67 | 0.795371 |
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Target: 5'- gGCGcucGCCGUGGAcgaUCACCUGGagcUUGCc-- -3' miRNA: 3'- -CGC---UGGCAUCU---AGUGGGCC---AACGacu -5' |
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| 30832 | 5' | -53.9 | NC_006552.1 | + | 21984 | 0.67 | 0.794407 |
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Target: 5'- cGCGACCGUgcAGcgccccaGUCGCCUGaucGCUGAc -3' miRNA: 3'- -CGCUGGCA--UC-------UAGUGGGCcaaCGACU- -5' |
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| 30832 | 5' | -53.9 | NC_006552.1 | + | 7088 | 0.68 | 0.734968 |
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Target: 5'- cGCGA-UGUAGGUCAUgCGGcgGUUGAu -3' miRNA: 3'- -CGCUgGCAUCUAGUGgGCCaaCGACU- -5' |
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| 30832 | 5' | -53.9 | NC_006552.1 | + | 25896 | 0.71 | 0.562276 |
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Target: 5'- gGCGACCGgcGAgcCAUCCGGUgaUGCg-- -3' miRNA: 3'- -CGCUGGCauCUa-GUGGGCCA--ACGacu -5' |
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| 30832 | 5' | -53.9 | NC_006552.1 | + | 12623 | 0.72 | 0.51989 |
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Target: 5'- cCGGCCGUAG-UCuGCCCGGc-GCUGGc -3' miRNA: 3'- cGCUGGCAUCuAG-UGGGCCaaCGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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