Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30834 | 3' | -52.7 | NC_006552.1 | + | 62489 | 0.66 | 0.879734 |
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Target: 5'- --aGgCGC--GCUGAAGguGAUCGCc -3' miRNA: 3'- gcaUgGCGaaCGACUUCguCUAGCGa -5' |
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| 30834 | 3' | -52.7 | NC_006552.1 | + | 2111 | 0.66 | 0.871866 |
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Target: 5'- aCGcUGCUGCUUGUc-GAGCAGAUCcCUg -3' miRNA: 3'- -GC-AUGGCGAACGacUUCGUCUAGcGA- -5' |
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| 30834 | 3' | -52.7 | NC_006552.1 | + | 40444 | 0.66 | 0.863742 |
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Target: 5'- cCGUugCGCUUGC----GCAGcGUCGCc -3' miRNA: 3'- -GCAugGCGAACGacuuCGUC-UAGCGa -5' |
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| 30834 | 3' | -52.7 | NC_006552.1 | + | 50169 | 0.66 | 0.851952 |
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Target: 5'- gCGUACUGCgcGCUGGucggcuaucugccGCAGcAUCGCa -3' miRNA: 3'- -GCAUGGCGaaCGACUu------------CGUC-UAGCGa -5' |
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| 30834 | 3' | -52.7 | NC_006552.1 | + | 11141 | 0.69 | 0.728219 |
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Target: 5'- uCGUACUGCUUGCcggacaccuccaUGAAGuCGGAUC-Cg -3' miRNA: 3'- -GCAUGGCGAACG------------ACUUC-GUCUAGcGa -5' |
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| 30834 | 3' | -52.7 | NC_006552.1 | + | 62517 | 0.7 | 0.617677 |
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Target: 5'- --cGCCGCcaggGCUucaucaccacGAAGCAGAUCGCc -3' miRNA: 3'- gcaUGGCGaa--CGA----------CUUCGUCUAGCGa -5' |
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| 30834 | 3' | -52.7 | NC_006552.1 | + | 53607 | 0.72 | 0.551253 |
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Target: 5'- gGUGCCGCcgGCUGAGGUGGAg-GCc -3' miRNA: 3'- gCAUGGCGaaCGACUUCGUCUagCGa -5' |
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| 30834 | 3' | -52.7 | NC_006552.1 | + | 10368 | 0.76 | 0.344348 |
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Target: 5'- uGUAUCGCaUGCUGGAGUucgugcaAGAUCGCc -3' miRNA: 3'- gCAUGGCGaACGACUUCG-------UCUAGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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