Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30840 | 3' | -59.4 | NC_006552.1 | + | 56705 | 0.66 | 0.54646 |
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Target: 5'- -cCUGUUCc--GCGUCGCGCGGGACc -3' miRNA: 3'- ucGACAAGcccUGCGGCGCGCUCUGc -5' |
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| 30840 | 3' | -59.4 | NC_006552.1 | + | 58613 | 0.66 | 0.54646 |
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Target: 5'- gGGCg--UC-GGugGCCGCGCcGGGCa -3' miRNA: 3'- -UCGacaAGcCCugCGGCGCGcUCUGc -5' |
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| 30840 | 3' | -59.4 | NC_006552.1 | + | 60843 | 0.68 | 0.466863 |
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Target: 5'- cGGCga-UCGGuuGugGgUGCGCGAGACGu -3' miRNA: 3'- -UCGacaAGCC--CugCgGCGCGCUCUGC- -5' |
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| 30840 | 3' | -59.4 | NC_006552.1 | + | 2760 | 0.69 | 0.39368 |
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Target: 5'- aGGCUGguucgagUGGGugGCCGaggGCGGGccGCGg -3' miRNA: 3'- -UCGACaa-----GCCCugCGGCg--CGCUC--UGC- -5' |
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| 30840 | 3' | -59.4 | NC_006552.1 | + | 8412 | 0.7 | 0.336066 |
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Target: 5'- -aCUGcUCGGGGCucagGCCGCGCcAGGCGc -3' miRNA: 3'- ucGACaAGCCCUG----CGGCGCGcUCUGC- -5' |
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| 30840 | 3' | -59.4 | NC_006552.1 | + | 35278 | 0.7 | 0.336066 |
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Target: 5'- -cCUGaUCGGGGCucauGCCGCGCaGGGCGu -3' miRNA: 3'- ucGACaAGCCCUG----CGGCGCGcUCUGC- -5' |
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| 30840 | 3' | -59.4 | NC_006552.1 | + | 55681 | 0.72 | 0.271398 |
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Target: 5'- cGGCUGccaggaaGGGGucgauCGCCGgGCGAGACGc -3' miRNA: 3'- -UCGACaag----CCCU-----GCGGCgCGCUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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