Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30844 | 5' | -53.5 | NC_006552.1 | + | 3836 | 0.66 | 0.825232 |
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Target: 5'- cGGUAuACCUGAGCu--GCCgAGGCUGa -3' miRNA: 3'- -CCAUcUGGACUUGcgcUGGgUUCGAC- -5' |
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| 30844 | 5' | -53.5 | NC_006552.1 | + | 54800 | 0.67 | 0.777342 |
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Target: 5'- --aGGACCUGAccagcgACGUGAUCCGccAGCa- -3' miRNA: 3'- ccaUCUGGACU------UGCGCUGGGU--UCGac -5' |
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| 30844 | 5' | -53.5 | NC_006552.1 | + | 35034 | 0.68 | 0.746721 |
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Target: 5'- aGGUccuGGGCCUGGACGauggGAaguUCCAGGCUc -3' miRNA: 3'- -CCA---UCUGGACUUGCg---CU---GGGUUCGAc -5' |
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| 30844 | 5' | -53.5 | NC_006552.1 | + | 58083 | 0.68 | 0.725709 |
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Target: 5'- ---uGGCCUGAGC-CGACCCAcgccaAGCa- -3' miRNA: 3'- ccauCUGGACUUGcGCUGGGU-----UCGac -5' |
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| 30844 | 5' | -53.5 | NC_006552.1 | + | 34130 | 0.68 | 0.725709 |
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Target: 5'- cGG-AGugCUcGGAUGCcGCCCAGGCUu -3' miRNA: 3'- -CCaUCugGA-CUUGCGcUGGGUUCGAc -5' |
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| 30844 | 5' | -53.5 | NC_006552.1 | + | 35922 | 0.69 | 0.693519 |
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Target: 5'- uGUAG-CUUGccuGCGCGGCCUggGCUu -3' miRNA: 3'- cCAUCuGGACu--UGCGCUGGGuuCGAc -5' |
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| 30844 | 5' | -53.5 | NC_006552.1 | + | 53507 | 0.69 | 0.671745 |
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Target: 5'- gGGaGGACCUGGuggaggcucaccGCGCGaugccggacGCCCAAGCc- -3' miRNA: 3'- -CCaUCUGGACU------------UGCGC---------UGGGUUCGac -5' |
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| 30844 | 5' | -53.5 | NC_006552.1 | + | 59589 | 0.72 | 0.488357 |
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Target: 5'- cGUGGAgCUGGACcCGGCUCAAGCg- -3' miRNA: 3'- cCAUCUgGACUUGcGCUGGGUUCGac -5' |
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| 30844 | 5' | -53.5 | NC_006552.1 | + | 45371 | 0.74 | 0.391626 |
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Target: 5'- cGGU--AUCUGGcaGCGCGGCCCGGGCUc -3' miRNA: 3'- -CCAucUGGACU--UGCGCUGGGUUCGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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