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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30849 | 5' | -52.4 | NC_006552.1 | + | 61096 | 0.66 | 0.859724 |
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Target: 5'- aGAUCACCGAcguacgcgucGAGCGGCUGCaGGa--- -3' miRNA: 3'- -CUAGUGGCU----------CUUGCUGGCGgUCauaa -5' |
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| 30849 | 5' | -52.4 | NC_006552.1 | + | 28729 | 0.66 | 0.851215 |
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Target: 5'- cGAUCugCGGGAAgcCGGCCGgCGGc--- -3' miRNA: 3'- -CUAGugGCUCUU--GCUGGCgGUCauaa -5' |
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| 30849 | 5' | -52.4 | NC_006552.1 | + | 29241 | 0.67 | 0.833492 |
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Target: 5'- cGGUCGCC-AGua-GGCCGCCAGg--- -3' miRNA: 3'- -CUAGUGGcUCuugCUGGCGGUCauaa -5' |
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| 30849 | 5' | -52.4 | NC_006552.1 | + | 4498 | 0.67 | 0.824297 |
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Target: 5'- gGcgCACCGA--GCGGCUGCCAGc--- -3' miRNA: 3'- -CuaGUGGCUcuUGCUGGCGGUCauaa -5' |
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| 30849 | 5' | -52.4 | NC_006552.1 | + | 51166 | 0.68 | 0.775399 |
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Target: 5'- aGUCGCCGAGAA-GGCCcgcgccaaagcGCCAGUcgUc -3' miRNA: 3'- cUAGUGGCUCUUgCUGG-----------CGGUCAuaA- -5' |
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| 30849 | 5' | -52.4 | NC_006552.1 | + | 63092 | 0.68 | 0.775399 |
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Target: 5'- cGUCGCCGAGAACuGCCGUgcgaUGGUGg- -3' miRNA: 3'- cUAGUGGCUCUUGcUGGCG----GUCAUaa -5' |
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| 30849 | 5' | -52.4 | NC_006552.1 | + | 31398 | 0.7 | 0.689826 |
|
Target: 5'- --gCACCGucGGCGgcGCCGCCAGUGg- -3' miRNA: 3'- cuaGUGGCucUUGC--UGGCGGUCAUaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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