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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30851 | 3' | -57.3 | NC_006552.1 | + | 35032 | 0.66 | 0.652238 |
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Target: 5'- cCAGGUCCugGgCCUGGacgaugggaAGUUccagGCUCGAc -3' miRNA: 3'- -GUCCAGGugCaGGACU---------UCAG----CGGGCU- -5' |
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| 30851 | 3' | -57.3 | NC_006552.1 | + | 57833 | 0.67 | 0.577663 |
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Target: 5'- aCAGGUCCACcgGUCgaGuGGUCaaGCUCGAu -3' miRNA: 3'- -GUCCAGGUG--CAGgaCuUCAG--CGGGCU- -5' |
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| 30851 | 3' | -57.3 | NC_006552.1 | + | 21787 | 0.69 | 0.505102 |
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Target: 5'- -cGGUCCAagccuggccgcCGUCCU--GGUCGCCCc- -3' miRNA: 3'- guCCAGGU-----------GCAGGAcuUCAGCGGGcu -5' |
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| 30851 | 3' | -57.3 | NC_006552.1 | + | 30358 | 0.73 | 0.295578 |
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Target: 5'- cCAGGUCgGCGUUgU--AGUUGCCCGAa -3' miRNA: 3'- -GUCCAGgUGCAGgAcuUCAGCGGGCU- -5' |
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| 30851 | 3' | -57.3 | NC_006552.1 | + | 15192 | 0.73 | 0.294862 |
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Target: 5'- aCAGGUCCACGgCCuUGAuggugccGGUCGCCUu- -3' miRNA: 3'- -GUCCAGGUGCaGG-ACU-------UCAGCGGGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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