Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30854 | 5' | -57.6 | NC_006552.1 | + | 63990 | 0.66 | 0.628775 |
Target: 5'- -aCCUGGCCGccaaGGGCCuGGc--CGGCAc -3' miRNA: 3'- cgGGACCGGU----UCCGG-CCuuuGCUGUa -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 61444 | 0.66 | 0.628775 |
Target: 5'- cGCCCgGGCCGuacggcaauacgAGuGCCGGGAAacCGAUc- -3' miRNA: 3'- -CGGGaCCGGU------------UC-CGGCCUUU--GCUGua -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 37963 | 0.66 | 0.596374 |
Target: 5'- gGCCUUGGCCAGGGCgucuugcACGGCc- -3' miRNA: 3'- -CGGGACCGGUUCCGgccuu--UGCUGua -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 21613 | 0.66 | 0.585621 |
Target: 5'- cGCCCUGGCCGAuGuccaguuucCCGGucGCGAUu- -3' miRNA: 3'- -CGGGACCGGUUcC---------GGCCuuUGCUGua -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 12955 | 0.67 | 0.573836 |
Target: 5'- gGCCUUGGCCuggcggugcguucGAGGCCaGcAGcCGACAUc -3' miRNA: 3'- -CGGGACCGG-------------UUCCGGcCuUU-GCUGUA- -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 17015 | 0.67 | 0.543061 |
Target: 5'- cGCCUc--CCGGGGCCGGAAGCcgaGCAUg -3' miRNA: 3'- -CGGGaccGGUUCCGGCCUUUGc--UGUA- -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 26187 | 0.67 | 0.522155 |
Target: 5'- cGCCCgcgccaguaGGCCAGGugcuuGCCGGu--CGGCAUg -3' miRNA: 3'- -CGGGa--------CCGGUUC-----CGGCCuuuGCUGUA- -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 7304 | 0.67 | 0.522155 |
Target: 5'- uGCCCgGGCCAGGuCCGGuucGAACuGAUAg -3' miRNA: 3'- -CGGGaCCGGUUCcGGCC---UUUG-CUGUa -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 8259 | 0.68 | 0.511818 |
Target: 5'- uGUCCaGGCUAcGGUCGGugGCGGCGc -3' miRNA: 3'- -CGGGaCCGGUuCCGGCCuuUGCUGUa -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 27354 | 0.68 | 0.461525 |
Target: 5'- aGCCCcacccgGGCCAGGaGCCGGcAGACcaGCAg -3' miRNA: 3'- -CGGGa-----CCGGUUC-CGGCC-UUUGc-UGUa -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 7882 | 0.68 | 0.461525 |
Target: 5'- uGCCCUGGCuCAcgguGGcGCCaGGAAACG-CGc -3' miRNA: 3'- -CGGGACCG-GU----UC-CGG-CCUUUGCuGUa -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 3575 | 0.68 | 0.461525 |
Target: 5'- gGUgCUGGCCGAGccGCCGGcgcCGGCAg -3' miRNA: 3'- -CGgGACCGGUUC--CGGCCuuuGCUGUa -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 64446 | 0.69 | 0.442145 |
Target: 5'- cGgCCUGGCCGuggugGGGCUGGuggaGACAUu -3' miRNA: 3'- -CgGGACCGGU-----UCCGGCCuuugCUGUA- -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 51030 | 0.69 | 0.432629 |
Target: 5'- aGCgCCUGGCCGAuGCCGGcGAUcccgaGACAg -3' miRNA: 3'- -CG-GGACCGGUUcCGGCCuUUG-----CUGUa -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 32180 | 0.7 | 0.39045 |
Target: 5'- aGCaCCUcGGCCGAcuucagcaccggcauGGUCGGgcGCGACAUa -3' miRNA: 3'- -CG-GGA-CCGGUU---------------CCGGCCuuUGCUGUA- -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 53476 | 0.7 | 0.381642 |
Target: 5'- aCCUUGGCCGAGGCauacaagcacuucacCGGGGAgGACc- -3' miRNA: 3'- cGGGACCGGUUCCG---------------GCCUUUgCUGua -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 46538 | 0.7 | 0.367828 |
Target: 5'- cGCgCCUGGCCGAGGagauagagcgcaUGGcAACGACGg -3' miRNA: 3'- -CG-GGACCGGUUCCg-----------GCCuUUGCUGUa -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 44990 | 0.7 | 0.364428 |
Target: 5'- aGCCCUccucgcucucugcucGGCCAGGGCCuGcucAGCGGCGg -3' miRNA: 3'- -CGGGA---------------CCGGUUCCGGcCu--UUGCUGUa -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 58196 | 0.71 | 0.352702 |
Target: 5'- gGCCCUGGCCGAagccacgcuGcGCCGGGuggcuAAgGGCGUg -3' miRNA: 3'- -CGGGACCGGUU---------C-CGGCCU-----UUgCUGUA- -5' |
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30854 | 5' | -57.6 | NC_006552.1 | + | 11735 | 0.71 | 0.344492 |
Target: 5'- gGCCUU-GCCGgugAGGCCGG-AGCGGCGa -3' miRNA: 3'- -CGGGAcCGGU---UCCGGCCuUUGCUGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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