Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30857 | 5' | -52.4 | NC_006552.1 | + | 17898 | 0.7 | 0.607548 |
Target: 5'- cAAGGCgGCCUGGCgaacgcuaucgGCCcGAGAGCUAu- -3' miRNA: 3'- -UUCUG-CGGACUG-----------CGGuUUCUCGAUuu -5' |
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30857 | 5' | -52.4 | NC_006552.1 | + | 46413 | 0.66 | 0.852475 |
Target: 5'- cGAGGgGCUUGGCGCCAccacccAGAGUg--- -3' miRNA: 3'- -UUCUgCGGACUGCGGUu-----UCUCGauuu -5' |
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30857 | 5' | -52.4 | NC_006552.1 | + | 60789 | 0.67 | 0.753862 |
Target: 5'- cAAGACGCUUGAUGCCGGccuGCa--- -3' miRNA: 3'- -UUCUGCGGACUGCGGUUucuCGauuu -5' |
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30857 | 5' | -52.4 | NC_006552.1 | + | 65086 | 0.68 | 0.721094 |
Target: 5'- uGGACGCCgccGGCGCCGgcaaacgaagcGGGAGCc--- -3' miRNA: 3'- uUCUGCGGa--CUGCGGU-----------UUCUCGauuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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