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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30859 | 5' | -55.4 | NC_006552.1 | + | 45125 | 0.66 | 0.700595 |
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Target: 5'- cGCCAGCaACagGCCGAGggGAuGGugCu- -3' miRNA: 3'- -UGGUCGcUG--CGGCUCuuCU-UCugGuu -5' |
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| 30859 | 5' | -55.4 | NC_006552.1 | + | 11358 | 0.67 | 0.645944 |
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Target: 5'- -aCGGCGGCcgagGuuGGGAAGAAGuCCAGg -3' miRNA: 3'- ugGUCGCUG----CggCUCUUCUUCuGGUU- -5' |
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| 30859 | 5' | -55.4 | NC_006552.1 | + | 26435 | 0.67 | 0.645944 |
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Target: 5'- gAUCAGCGacgGCGCCGAcuccuGggGGCCGc -3' miRNA: 3'- -UGGUCGC---UGCGGCUcuu--CuuCUGGUu -5' |
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| 30859 | 5' | -55.4 | NC_006552.1 | + | 54252 | 0.68 | 0.612915 |
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Target: 5'- aACCAGCGcgcccuGCGCCucccAGGAGuuGACCAc -3' miRNA: 3'- -UGGUCGC------UGCGGc---UCUUCuuCUGGUu -5' |
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| 30859 | 5' | -55.4 | NC_006552.1 | + | 26912 | 0.71 | 0.415722 |
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Target: 5'- gGCCAGCGGCaCCaGGGAAGucguGGCCAu -3' miRNA: 3'- -UGGUCGCUGcGG-CUCUUCuu--CUGGUu -5' |
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| 30859 | 5' | -55.4 | NC_006552.1 | + | 52016 | 0.72 | 0.38812 |
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Target: 5'- cGCCAGCaacuCGCCGAGcAGGAGACa-- -3' miRNA: 3'- -UGGUCGcu--GCGGCUCuUCUUCUGguu -5' |
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| 30859 | 5' | -55.4 | NC_006552.1 | + | 51531 | 0.72 | 0.361749 |
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Target: 5'- gGCCAGaucauCGACGCCGAGgcGAagcGGAUCAc -3' miRNA: 3'- -UGGUC-----GCUGCGGCUCuuCU---UCUGGUu -5' |
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| 30859 | 5' | -55.4 | NC_006552.1 | + | 53256 | 0.75 | 0.255681 |
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Target: 5'- cAUCAGCGAgGCCGAGGcucuGGAGGGCUu- -3' miRNA: 3'- -UGGUCGCUgCGGCUCU----UCUUCUGGuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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