Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30862 | 5' | -55.5 | NC_006552.1 | + | 28019 | 0.66 | 0.779893 |
Target: 5'- cGGcGAGUACGGCggcgcgccggucuugGUCACCACCaGCUu -3' miRNA: 3'- cCCcCUCAUGUUG---------------UAGUGGUGG-CGGc -5' |
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30862 | 5' | -55.5 | NC_006552.1 | + | 59341 | 0.67 | 0.725348 |
Target: 5'- --cGGua-ACAGCGUCAgcCCGCCGCCGa -3' miRNA: 3'- cccCCucaUGUUGUAGU--GGUGGCGGC- -5' |
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30862 | 5' | -55.5 | NC_006552.1 | + | 55498 | 0.67 | 0.683177 |
Target: 5'- aGGGGcggcggugaacGAGUcACugGACGUCACC-CCGUCGa -3' miRNA: 3'- -CCCC-----------CUCA-UG--UUGUAGUGGuGGCGGC- -5' |
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30862 | 5' | -55.5 | NC_006552.1 | + | 33717 | 0.68 | 0.648869 |
Target: 5'- -cGGGAacucgaacgccACGACAUCGCCaACCGCCa -3' miRNA: 3'- ccCCCUca---------UGUUGUAGUGG-UGGCGGc -5' |
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30862 | 5' | -55.5 | NC_006552.1 | + | 63747 | 0.68 | 0.640256 |
Target: 5'- ---cGAGcaggACAACAUgACCGCCGCCa -3' miRNA: 3'- ccccCUCa---UGUUGUAgUGGUGGCGGc -5' |
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30862 | 5' | -55.5 | NC_006552.1 | + | 60144 | 0.71 | 0.462831 |
Target: 5'- uGGcGGGAgaaaGUGCGGCAgaUCGCCGgccagcacuuCCGCCGg -3' miRNA: 3'- -CC-CCCU----CAUGUUGU--AGUGGU----------GGCGGC- -5' |
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30862 | 5' | -55.5 | NC_006552.1 | + | 2882 | 0.77 | 0.205158 |
Target: 5'- -cGGGAGaACAGCAuggguucgucaUCAUCACCGCCGa -3' miRNA: 3'- ccCCCUCaUGUUGU-----------AGUGGUGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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