Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30865 | 3' | -54.8 | NC_006552.1 | + | 13393 | 0.66 | 0.775791 |
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Target: 5'- gACUCCGGCAcgcucaUGCCGcgCUcacggaacagcgUCgcuaGGUCg -3' miRNA: 3'- -UGAGGCCGU------ACGGCaaGA------------AGa---CCAGg -5' |
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| 30865 | 3' | -54.8 | NC_006552.1 | + | 19020 | 0.66 | 0.775791 |
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Target: 5'- uCUCCGGCuUGCCGggggcgcUCUUUUccGGUUUg -3' miRNA: 3'- uGAGGCCGuACGGCa------AGAAGA--CCAGG- -5' |
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| 30865 | 3' | -54.8 | NC_006552.1 | + | 49167 | 0.68 | 0.67091 |
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Target: 5'- cCUCCGGC-UGcCCGcaCUgcggcggcCUGGUCCg -3' miRNA: 3'- uGAGGCCGuAC-GGCaaGAa-------GACCAGG- -5' |
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| 30865 | 3' | -54.8 | NC_006552.1 | + | 35693 | 0.68 | 0.649155 |
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Target: 5'- cUUCUGGaucgGUGCCag-UUUCUGGUCCa -3' miRNA: 3'- uGAGGCCg---UACGGcaaGAAGACCAGG- -5' |
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| 30865 | 3' | -54.8 | NC_006552.1 | + | 48843 | 0.68 | 0.649155 |
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Target: 5'- cCUCCGGCAUGgCGgUgaUCUGG-CUa -3' miRNA: 3'- uGAGGCCGUACgGCaAgaAGACCaGG- -5' |
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| 30865 | 3' | -54.8 | NC_006552.1 | + | 62968 | 0.68 | 0.63825 |
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Target: 5'- aACUggCCGGC--GCCGUUCUUCUG--CCg -3' miRNA: 3'- -UGA--GGCCGuaCGGCAAGAAGACcaGG- -5' |
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| 30865 | 3' | -54.8 | NC_006552.1 | + | 42348 | 0.69 | 0.616432 |
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Target: 5'- uCUUCGGUuugGUGCUGggCUUCUGgGUCa -3' miRNA: 3'- uGAGGCCG---UACGGCaaGAAGAC-CAGg -5' |
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| 30865 | 3' | -54.8 | NC_006552.1 | + | 5146 | 0.69 | 0.583828 |
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Target: 5'- uGCaUUGcGCAUGCCGcUCcugcugUCUGGUCCa -3' miRNA: 3'- -UGaGGC-CGUACGGCaAGa-----AGACCAGG- -5' |
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| 30865 | 3' | -54.8 | NC_006552.1 | + | 10945 | 0.72 | 0.458884 |
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Target: 5'- cUUCCGGCA-GCCGUUCa-CUGGUg- -3' miRNA: 3'- uGAGGCCGUaCGGCAAGaaGACCAgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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