Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30867 | 5' | -55.3 | NC_006552.1 | + | 22046 | 0.66 | 0.70841 |
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Target: 5'- cUGGCGGCCUGGCCAgUgCUGuCGcUGa -3' miRNA: 3'- uACCGUUGGACUGGU-GgGAC-GCaAUa -5' |
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| 30867 | 5' | -55.3 | NC_006552.1 | + | 56299 | 0.66 | 0.686457 |
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Target: 5'- --aGCAgcGCCUGACCGCCCU-CGa--- -3' miRNA: 3'- uacCGU--UGGACUGGUGGGAcGCaaua -5' |
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| 30867 | 5' | -55.3 | NC_006552.1 | + | 34151 | 0.67 | 0.641997 |
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Target: 5'- -aGGCuugcgccagAGCCUGGCCGCCCaGCa---- -3' miRNA: 3'- uaCCG---------UUGGACUGGUGGGaCGcaaua -5' |
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| 30867 | 5' | -55.3 | NC_006552.1 | + | 25895 | 0.67 | 0.619667 |
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Target: 5'- -cGGCGACCggcgaGCCAUCCggugaUGCGUUGg -3' miRNA: 3'- uaCCGUUGGac---UGGUGGG-----ACGCAAUa -5' |
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| 30867 | 5' | -55.3 | NC_006552.1 | + | 41854 | 0.67 | 0.619667 |
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Target: 5'- -cGGCAuuggggucgaaGCCUgGGCCGCCCUuGaCGUUGa -3' miRNA: 3'- uaCCGU-----------UGGA-CUGGUGGGA-C-GCAAUa -5' |
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| 30867 | 5' | -55.3 | NC_006552.1 | + | 39518 | 0.67 | 0.615204 |
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Target: 5'- -cGGCAAcuuCCUGGCCGCCCacgucgccguucgGCGUc-- -3' miRNA: 3'- uaCCGUU---GGACUGGUGGGa------------CGCAaua -5' |
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| 30867 | 5' | -55.3 | NC_006552.1 | + | 18293 | 0.67 | 0.597382 |
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Target: 5'- -cGGCAacACCUGGCCucGCCCggGCGc--- -3' miRNA: 3'- uaCCGU--UGGACUGG--UGGGa-CGCaaua -5' |
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| 30867 | 5' | -55.3 | NC_006552.1 | + | 35420 | 0.69 | 0.489107 |
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Target: 5'- gAUGGCAGCCUgGGCCGCagCCUucGCGUc-- -3' miRNA: 3'- -UACCGUUGGA-CUGGUG--GGA--CGCAaua -5' |
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| 30867 | 5' | -55.3 | NC_006552.1 | + | 63362 | 0.71 | 0.40951 |
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Target: 5'- -gGGCAACCcGGuCCGCaCCUGCGUc-- -3' miRNA: 3'- uaCCGUUGGaCU-GGUG-GGACGCAaua -5' |
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| 30867 | 5' | -55.3 | NC_006552.1 | + | 35831 | 0.73 | 0.311914 |
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Target: 5'- cAUGGCAGCCUGAgCCGCcgccaguugguuguCCUGCGa--- -3' miRNA: 3'- -UACCGUUGGACU-GGUG--------------GGACGCaaua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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