Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30879 | 5' | -54.3 | NC_006552.1 | + | 2078 | 0.68 | 0.693519 |
Target: 5'- cGCGgccugcugaucGAAC-UCGACGCCGGCggCAc -3' miRNA: 3'- cCGCa----------CUUGuAGCUGCGGCCGuaGUu -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 33758 | 0.66 | 0.816019 |
Target: 5'- cGGCGgcauugcuguUGAGCAga-ACGCCGGCcgCGu -3' miRNA: 3'- -CCGC----------ACUUGUagcUGCGGCCGuaGUu -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 31992 | 0.66 | 0.806613 |
Target: 5'- cGGCGUGAAUcucCGGCuGCgCGGUGUCc- -3' miRNA: 3'- -CCGCACUUGua-GCUG-CG-GCCGUAGuu -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 59011 | 0.66 | 0.806613 |
Target: 5'- cGGCGccGAGCAUC-ACGCCcuCAUCGAg -3' miRNA: 3'- -CCGCa-CUUGUAGcUGCGGccGUAGUU- -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 27856 | 0.67 | 0.76727 |
Target: 5'- -cCGUccACcUUGAUGCCGGCGUCGAu -3' miRNA: 3'- ccGCAcuUGuAGCUGCGGCCGUAGUU- -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 36064 | 0.67 | 0.766255 |
Target: 5'- aGGCGUcuuGCccuguugGUCGccaccagccgaaGCGCCGGCGUCAc -3' miRNA: 3'- -CCGCAcu-UG-------UAGC------------UGCGGCCGUAGUu -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 10850 | 0.67 | 0.75706 |
Target: 5'- cGGCGcUGccGACucuUCGGCuGCCGGCuuGUCGAu -3' miRNA: 3'- -CCGC-AC--UUGu--AGCUG-CGGCCG--UAGUU- -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 59586 | 0.67 | 0.75706 |
Target: 5'- cGGCGUGGAgcUgGAC-CCGGC-UCAAg -3' miRNA: 3'- -CCGCACUUguAgCUGcGGCCGuAGUU- -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 49716 | 0.67 | 0.746721 |
Target: 5'- cGGUGcUGAugAUCGAgGaaGGCAUUAc -3' miRNA: 3'- -CCGC-ACUugUAGCUgCggCCGUAGUu -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 24246 | 0.68 | 0.703246 |
Target: 5'- cGGCG-GAacGCAUCGGuugugguCGCCGGCAc--- -3' miRNA: 3'- -CCGCaCU--UGUAGCU-------GCGGCCGUaguu -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 34614 | 0.76 | 0.286044 |
Target: 5'- cGGCGcaacgccagUGAugGCAUCGGCGCUGGCAaCGAa -3' miRNA: 3'- -CCGC---------ACU--UGUAGCUGCGGCCGUaGUU- -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 60871 | 0.68 | 0.693519 |
Target: 5'- uGGacugaGUGAACAUgugcggcgcacCGGCGCUGGCAuaUCGGg -3' miRNA: 3'- -CCg----CACUUGUA-----------GCUGCGGCCGU--AGUU- -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 22599 | 0.68 | 0.671745 |
Target: 5'- uGGCGUaccGGGCGUCGccgauggcgacAUGCaCGGCGUCGc -3' miRNA: 3'- -CCGCA---CUUGUAGC-----------UGCG-GCCGUAGUu -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 20827 | 0.69 | 0.627834 |
Target: 5'- cGGCGUG-GCGUCGaACGuaGGCAacUCGGg -3' miRNA: 3'- -CCGCACuUGUAGC-UGCggCCGU--AGUU- -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 31505 | 0.69 | 0.594903 |
Target: 5'- cGGCGUGuAU----GCGCCGGUAUCGAc -3' miRNA: 3'- -CCGCACuUGuagcUGCGGCCGUAGUU- -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 50496 | 0.7 | 0.583977 |
Target: 5'- cGCG-GAACAaccagccaCGGCGCCGGCAgccgUCAAg -3' miRNA: 3'- cCGCaCUUGUa-------GCUGCGGCCGU----AGUU- -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 2052 | 0.71 | 0.530107 |
Target: 5'- aGGCGcuUGAGC----GCGCCGGCGUCGu -3' miRNA: 3'- -CCGC--ACUUGuagcUGCGGCCGUAGUu -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 19725 | 0.71 | 0.488357 |
Target: 5'- cGGCGUcGGccACGUUGGCGCUGGCcgCu- -3' miRNA: 3'- -CCGCA-CU--UGUAGCUGCGGCCGuaGuu -5' |
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30879 | 5' | -54.3 | NC_006552.1 | + | 36159 | 0.73 | 0.382656 |
Target: 5'- cGGCG---GCAUCGGCGCUGGCcUCGc -3' miRNA: 3'- -CCGCacuUGUAGCUGCGGCCGuAGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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