Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30881 | 5' | -57.4 | NC_006552.1 | + | 34045 | 0.66 | 0.622998 |
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Target: 5'- uUGGAgCaGCCGAggaucgcGCCGAUCAgcagUUCCAGc -3' miRNA: 3'- -ACCUgG-CGGCUa------CGGCUGGU----AAGGUU- -5' |
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| 30881 | 5' | -57.4 | NC_006552.1 | + | 61639 | 0.66 | 0.612165 |
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Target: 5'- cUGGAUgcCCGAggccGCCGGCCGgacgUCCAAc -3' miRNA: 3'- -ACCUGgcGGCUa---CGGCUGGUa---AGGUU- -5' |
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| 30881 | 5' | -57.4 | NC_006552.1 | + | 14538 | 0.66 | 0.611082 |
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Target: 5'- cGGGCCGCCauccacuGGUGauGGCCG-UCCAGc -3' miRNA: 3'- aCCUGGCGG-------CUACggCUGGUaAGGUU- -5' |
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| 30881 | 5' | -57.4 | NC_006552.1 | + | 32137 | 0.67 | 0.579801 |
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Target: 5'- cGGcUUGCCGGUGCaugcgcGCCAUUCCGg -3' miRNA: 3'- aCCuGGCGGCUACGgc----UGGUAAGGUu -5' |
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| 30881 | 5' | -57.4 | NC_006552.1 | + | 21177 | 0.67 | 0.569087 |
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Target: 5'- -cGGCCGCguCGGUGCCGGCaCAU-CCGc -3' miRNA: 3'- acCUGGCG--GCUACGGCUG-GUAaGGUu -5' |
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| 30881 | 5' | -57.4 | NC_006552.1 | + | 2663 | 0.67 | 0.526799 |
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Target: 5'- gGGACCGCgCGgcGCCGAUCAa----- -3' miRNA: 3'- aCCUGGCG-GCuaCGGCUGGUaagguu -5' |
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| 30881 | 5' | -57.4 | NC_006552.1 | + | 39596 | 0.68 | 0.506093 |
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Target: 5'- gGGACaacagCGCCGG-GCCGAUCAggucUUCCAc -3' miRNA: 3'- aCCUG-----GCGGCUaCGGCUGGU----AAGGUu -5' |
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| 30881 | 5' | -57.4 | NC_006552.1 | + | 51030 | 0.68 | 0.485743 |
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Target: 5'- aGcGCCugGCCGAUGCCGGCgAUcCCGAg -3' miRNA: 3'- aCcUGG--CGGCUACGGCUGgUAaGGUU- -5' |
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| 30881 | 5' | -57.4 | NC_006552.1 | + | 29552 | 0.69 | 0.446271 |
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Target: 5'- cGGAUCGCCGGUGgCGguACCAUggaaucgcugaaUCCGc -3' miRNA: 3'- aCCUGGCGGCUACgGC--UGGUA------------AGGUu -5' |
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| 30881 | 5' | -57.4 | NC_006552.1 | + | 2787 | 0.69 | 0.446271 |
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Target: 5'- cGGGCCGCgGAaacaGCCGuuCCAUaUCCAAc -3' miRNA: 3'- aCCUGGCGgCUa---CGGCu-GGUA-AGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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