Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30885 | 3' | -48.7 | NC_006552.1 | + | 22329 | 0.66 | 0.984792 |
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Target: 5'- cCGGUCgGUCGAUAGccucauccGACACCGc -3' miRNA: 3'- -GCCAGgUAGCUGUUauac----UUGUGGCa -5' |
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| 30885 | 3' | -48.7 | NC_006552.1 | + | 61447 | 0.66 | 0.982734 |
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Target: 5'- cCGGgCCGUaCGGCAAUAcGAGUGCCGg -3' miRNA: 3'- -GCCaGGUA-GCUGUUAUaCUUGUGGCa -5' |
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| 30885 | 3' | -48.7 | NC_006552.1 | + | 64663 | 0.66 | 0.980474 |
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Target: 5'- cCGGUCagcuucgCGACAgaGUAUgugccGAGCACCGg -3' miRNA: 3'- -GCCAGgua----GCUGU--UAUA-----CUUGUGGCa -5' |
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| 30885 | 3' | -48.7 | NC_006552.1 | + | 7314 | 0.67 | 0.975301 |
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Target: 5'- aGGUCCGguUCGAacuGAUAgGAGCGCUGc -3' miRNA: 3'- gCCAGGU--AGCUg--UUAUaCUUGUGGCa -5' |
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| 30885 | 3' | -48.7 | NC_006552.1 | + | 14537 | 0.67 | 0.969193 |
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Target: 5'- gCGGgccgCCAUCcACug-GUGAugGCCGUc -3' miRNA: 3'- -GCCa---GGUAGcUGuuaUACUugUGGCA- -5' |
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| 30885 | 3' | -48.7 | NC_006552.1 | + | 30108 | 0.67 | 0.965409 |
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Target: 5'- uGGUCCA-CGACGGUGcccGAGacgguucCACCGUa -3' miRNA: 3'- gCCAGGUaGCUGUUAUa--CUU-------GUGGCA- -5' |
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| 30885 | 3' | -48.7 | NC_006552.1 | + | 26263 | 0.68 | 0.953897 |
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Target: 5'- cCGGUagCGUCGGCAg---GAugACCGUg -3' miRNA: 3'- -GCCAg-GUAGCUGUuauaCUugUGGCA- -5' |
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| 30885 | 3' | -48.7 | NC_006552.1 | + | 25224 | 0.68 | 0.939522 |
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Target: 5'- gCGGcgCCGUUGACGu--UGAGCugCGa -3' miRNA: 3'- -GCCa-GGUAGCUGUuauACUUGugGCa -5' |
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| 30885 | 3' | -48.7 | NC_006552.1 | + | 42439 | 0.75 | 0.649255 |
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Target: 5'- uGGUCCAUCGACAGg--GAAaGCUGg -3' miRNA: 3'- gCCAGGUAGCUGUUauaCUUgUGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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