Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30888 | 3' | -54.4 | NC_006552.1 | + | 23663 | 0.66 | 0.790536 |
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Target: 5'- aCGGCCaGUag-AACGGUCCAcugGCCAc -3' miRNA: 3'- -GUCGGcCGcuaUUGCCAGGUa--UGGUu -5' |
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| 30888 | 3' | -54.4 | NC_006552.1 | + | 27227 | 0.67 | 0.72878 |
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Target: 5'- uGGUCGGCaucGGCGGUCCAacaugcagcgacUGCCAc -3' miRNA: 3'- gUCGGCCGcuaUUGCCAGGU------------AUGGUu -5' |
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| 30888 | 3' | -54.4 | NC_006552.1 | + | 59682 | 0.67 | 0.718077 |
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Target: 5'- cCGGCCaacGGCGGUGAuCGGcaaUCCGcccUACCAGg -3' miRNA: 3'- -GUCGG---CCGCUAUU-GCC---AGGU---AUGGUU- -5' |
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| 30888 | 3' | -54.4 | NC_006552.1 | + | 4126 | 0.67 | 0.718077 |
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Target: 5'- cCAGCCGcuGCGGUAguaGCGGU-UGUACCGAu -3' miRNA: 3'- -GUCGGC--CGCUAU---UGCCAgGUAUGGUU- -5' |
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| 30888 | 3' | -54.4 | NC_006552.1 | + | 5020 | 0.67 | 0.696423 |
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Target: 5'- aGGuuGGCGGUGGCG-UCCugguacagGUGCCGAu -3' miRNA: 3'- gUCggCCGCUAUUGCcAGG--------UAUGGUU- -5' |
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| 30888 | 3' | -54.4 | NC_006552.1 | + | 24733 | 0.68 | 0.678914 |
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Target: 5'- cCAGCCGGCGccgggccugcuCGGUCaaccuUAUGCCAAc -3' miRNA: 3'- -GUCGGCCGCuauu-------GCCAG-----GUAUGGUU- -5' |
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| 30888 | 3' | -54.4 | NC_006552.1 | + | 35392 | 0.68 | 0.641381 |
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Target: 5'- uGGCCGGCGGUu-CGGUCUugGCUu- -3' miRNA: 3'- gUCGGCCGCUAuuGCCAGGuaUGGuu -5' |
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| 30888 | 3' | -54.4 | NC_006552.1 | + | 38392 | 0.69 | 0.608167 |
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Target: 5'- cCGGCCGGCGcgGAaguuGUCCAUgauccgGCCAAu -3' miRNA: 3'- -GUCGGCCGCuaUUgc--CAGGUA------UGGUU- -5' |
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| 30888 | 3' | -54.4 | NC_006552.1 | + | 24820 | 0.7 | 0.564213 |
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Target: 5'- aCAGCaUGGgGAUGuUGGUCUGUGCCAc -3' miRNA: 3'- -GUCG-GCCgCUAUuGCCAGGUAUGGUu -5' |
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| 30888 | 3' | -54.4 | NC_006552.1 | + | 26811 | 0.7 | 0.542536 |
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Target: 5'- cCGGCCGGCGAca--GGUCUAUguucACCGc -3' miRNA: 3'- -GUCGGCCGCUauugCCAGGUA----UGGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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