Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30896 | 5' | -52.5 | NC_006552.1 | + | 14007 | 0.66 | 0.876327 |
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Target: 5'- -cUGCuGCUGUUcgagauaCCGgaguAGCUGGCUGCg -3' miRNA: 3'- cuACGcCGAUAGa------GGU----UCGACUGACG- -5' |
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| 30896 | 5' | -52.5 | NC_006552.1 | + | 21481 | 0.66 | 0.860002 |
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Target: 5'- -cUGCaGGCUcgcUCUCCAgcggguAGCUGuaggucGCUGCg -3' miRNA: 3'- cuACG-CCGAu--AGAGGU------UCGAC------UGACG- -5' |
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| 30896 | 5' | -52.5 | NC_006552.1 | + | 21125 | 0.66 | 0.860002 |
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Target: 5'- aGGU-CGGCUGcUCgcCCAGGUUGACggGCg -3' miRNA: 3'- -CUAcGCCGAU-AGa-GGUUCGACUGa-CG- -5' |
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| 30896 | 5' | -52.5 | NC_006552.1 | + | 13891 | 0.67 | 0.824441 |
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Target: 5'- cGUGUGGCUugCUCCAcGGCcGcCUGCu -3' miRNA: 3'- cUACGCCGAuaGAGGU-UCGaCuGACG- -5' |
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| 30896 | 5' | -52.5 | NC_006552.1 | + | 4738 | 0.67 | 0.795521 |
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Target: 5'- --gGCGGC-GUCUCCugcaccAGCUucggcggugccGGCUGCg -3' miRNA: 3'- cuaCGCCGaUAGAGGu-----UCGA-----------CUGACG- -5' |
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| 30896 | 5' | -52.5 | NC_006552.1 | + | 5678 | 0.67 | 0.795521 |
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Target: 5'- cGAUGCgGGCagcgCUCUcGGCUGAUUcGCg -3' miRNA: 3'- -CUACG-CCGaua-GAGGuUCGACUGA-CG- -5' |
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| 30896 | 5' | -52.5 | NC_006552.1 | + | 10830 | 0.73 | 0.490472 |
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Target: 5'- --gGCGGUUGUUcgCCGGGCUGcggcGCUGCc -3' miRNA: 3'- cuaCGCCGAUAGa-GGUUCGAC----UGACG- -5' |
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| 30896 | 5' | -52.5 | NC_006552.1 | + | 45041 | 0.77 | 0.28376 |
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Target: 5'- cGAUGCGGUguUCUCCu-GCUGGCgUGCc -3' miRNA: 3'- -CUACGCCGauAGAGGuuCGACUG-ACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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