Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30897 | 5' | -54.1 | NC_006552.1 | + | 829 | 0.66 | 0.839798 |
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Target: 5'- gGCCGGGuauAGGUCAuuguccAGUacUCugcGCGCCUg -3' miRNA: 3'- gCGGCUCu--UCCAGU------UCA--AGc--UGCGGA- -5' |
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| 30897 | 5' | -54.1 | NC_006552.1 | + | 14287 | 0.66 | 0.803345 |
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Target: 5'- uGCCGG--GGGUgAAGUauUCGGCGCg- -3' miRNA: 3'- gCGGCUcuUCCAgUUCA--AGCUGCGga -5' |
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| 30897 | 5' | -54.1 | NC_006552.1 | + | 50029 | 0.67 | 0.793766 |
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Target: 5'- gCGCUGAcu-GGUaCAGGUUCuACGCCa -3' miRNA: 3'- -GCGGCUcuuCCA-GUUCAAGcUGCGGa -5' |
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| 30897 | 5' | -54.1 | NC_006552.1 | + | 11976 | 0.67 | 0.77412 |
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Target: 5'- uGCUgGGGAAGGUgGAGUaguccaccgucUCGGCGUCc -3' miRNA: 3'- gCGG-CUCUUCCAgUUCA-----------AGCUGCGGa -5' |
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| 30897 | 5' | -54.1 | NC_006552.1 | + | 57844 | 0.68 | 0.733185 |
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Target: 5'- gGUCGAGu-GGUCAAGcUCGAUGgCa -3' miRNA: 3'- gCGGCUCuuCCAGUUCaAGCUGCgGa -5' |
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| 30897 | 5' | -54.1 | NC_006552.1 | + | 41215 | 0.68 | 0.722674 |
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Target: 5'- aGCCGAGcugacgcgccuuGAGGaUCAGGUUgCGGUGCCa -3' miRNA: 3'- gCGGCUC------------UUCC-AGUUCAA-GCUGCGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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