Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30899 | 5' | -59.9 | NC_006552.1 | + | 24789 | 0.66 | 0.512621 |
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Target: 5'- aGCaCCUGGGcGCGGgugaugACGCCGgagaaCAGCa -3' miRNA: 3'- -CG-GGACCC-CGUCa-----UGUGGCga---GUCGa -5' |
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| 30899 | 5' | -59.9 | NC_006552.1 | + | 32080 | 0.66 | 0.50252 |
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Target: 5'- aGCCCggucgGGGGUug-GCACCagGCgCAGCg -3' miRNA: 3'- -CGGGa----CCCCGucaUGUGG--CGaGUCGa -5' |
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| 30899 | 5' | -59.9 | NC_006552.1 | + | 48760 | 0.66 | 0.50252 |
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Target: 5'- gGCCCaGGuGGCcguugAGUACcguCCGCUCcGCg -3' miRNA: 3'- -CGGGaCC-CCG-----UCAUGu--GGCGAGuCGa -5' |
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| 30899 | 5' | -59.9 | NC_006552.1 | + | 56436 | 0.66 | 0.496501 |
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Target: 5'- cGCCCUGuGGGCGauucaaccaugccucGUGgGCCGCccugUCAgGCa -3' miRNA: 3'- -CGGGAC-CCCGU---------------CAUgUGGCG----AGU-CGa -5' |
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| 30899 | 5' | -59.9 | NC_006552.1 | + | 11974 | 0.66 | 0.482586 |
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Target: 5'- uGUgCUGGGGaagguggagUAGUcCACCGuCUCGGCg -3' miRNA: 3'- -CGgGACCCC---------GUCAuGUGGC-GAGUCGa -5' |
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| 30899 | 5' | -59.9 | NC_006552.1 | + | 55293 | 0.67 | 0.434528 |
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Target: 5'- aGUCC-GGGGCc-UAC-CCGUUCAGCg -3' miRNA: 3'- -CGGGaCCCCGucAUGuGGCGAGUCGa -5' |
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| 30899 | 5' | -59.9 | NC_006552.1 | + | 58345 | 0.71 | 0.235487 |
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Target: 5'- gGCCUcgcgGGGGCAGUuuCGCCGUggcCAGCc -3' miRNA: 3'- -CGGGa---CCCCGUCAu-GUGGCGa--GUCGa -5' |
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| 30899 | 5' | -59.9 | NC_006552.1 | + | 50156 | 0.72 | 0.207309 |
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Target: 5'- cGCCCUGGaGCAGgcguacugcGCGCUGgUCGGCUa -3' miRNA: 3'- -CGGGACCcCGUCa--------UGUGGCgAGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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