Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30906 | 3' | -58 | NC_006552.1 | + | 52274 | 0.66 | 0.594437 |
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Target: 5'- cUGCCGGGCGUGAUCGUcgaaaAGGc- -3' miRNA: 3'- aGCGGCUCGCGCUAGCGaacg-UCCuu -5' |
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| 30906 | 3' | -58 | NC_006552.1 | + | 3581 | 0.67 | 0.541132 |
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Target: 5'- -gGCCGAGcCGCcggCGCcgGCAGGAc -3' miRNA: 3'- agCGGCUC-GCGcuaGCGaaCGUCCUu -5' |
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| 30906 | 3' | -58 | NC_006552.1 | + | 52004 | 0.67 | 0.541132 |
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Target: 5'- cUUGCCGcGGCGCGccagcaacUCGCcgaGCAGGAGa -3' miRNA: 3'- -AGCGGC-UCGCGCu-------AGCGaa-CGUCCUU- -5' |
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| 30906 | 3' | -58 | NC_006552.1 | + | 60833 | 0.67 | 0.50991 |
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Target: 5'- aCGgCGAGCccgGCGAUCGgUUGUGGGu- -3' miRNA: 3'- aGCgGCUCG---CGCUAGCgAACGUCCuu -5' |
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| 30906 | 3' | -58 | NC_006552.1 | + | 39605 | 0.68 | 0.449941 |
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Target: 5'- gCGCCGGGC-CGAUCaggucuuccacuGCggGCGGGAu -3' miRNA: 3'- aGCGGCUCGcGCUAG------------CGaaCGUCCUu -5' |
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| 30906 | 3' | -58 | NC_006552.1 | + | 33856 | 0.7 | 0.36786 |
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Target: 5'- cCGCCGAGCGgGAUgGuCUUGUAcuGGGc -3' miRNA: 3'- aGCGGCUCGCgCUAgC-GAACGU--CCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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