Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30909 | 5' | -54.6 | NC_006552.1 | + | 26265 | 0.66 | 0.829046 |
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Target: 5'- gGUAgCGUCGGCAg--GaUGACCGuGGc -3' miRNA: 3'- gCAUgGCAGUCGUagaC-GCUGGCuCC- -5' |
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| 30909 | 5' | -54.6 | NC_006552.1 | + | 7544 | 0.66 | 0.82816 |
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Target: 5'- cCGUACCG-CGGCGUCcuucaguucgcccUGCGcCUGcGGGa -3' miRNA: 3'- -GCAUGGCaGUCGUAG-------------ACGCuGGC-UCC- -5' |
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| 30909 | 5' | -54.6 | NC_006552.1 | + | 45109 | 0.66 | 0.820093 |
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Target: 5'- gGUaGCCcUCAGCGUUcGCcagcaacaGGCCGAGGg -3' miRNA: 3'- gCA-UGGcAGUCGUAGaCG--------CUGGCUCC- -5' |
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| 30909 | 5' | -54.6 | NC_006552.1 | + | 44445 | 0.66 | 0.81095 |
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Target: 5'- aCGUGCUGUucgagCAGCAaCUGCGcuGCCGGc- -3' miRNA: 3'- -GCAUGGCA-----GUCGUaGACGC--UGGCUcc -5' |
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| 30909 | 5' | -54.6 | NC_006552.1 | + | 19002 | 0.66 | 0.800684 |
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Target: 5'- --cACCGUCGGCAaaaaacaUCUcCGgcuuGCCGGGGg -3' miRNA: 3'- gcaUGGCAGUCGU-------AGAcGC----UGGCUCC- -5' |
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| 30909 | 5' | -54.6 | NC_006552.1 | + | 39424 | 0.68 | 0.721779 |
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Target: 5'- -aUACCG-CAcGCgAUCUGCGGCCGGa- -3' miRNA: 3'- gcAUGGCaGU-CG-UAGACGCUGGCUcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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