Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30910 | 3' | -53.7 | NC_006552.1 | + | 2583 | 0.66 | 0.844232 |
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Target: 5'- gGCcguGACGACGCUacggCUCGAAGG-CGa -3' miRNA: 3'- -CGu--CUGCUGCGGaaa-GGGCUUCCaGU- -5' |
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| 30910 | 3' | -53.7 | NC_006552.1 | + | 58799 | 0.67 | 0.808106 |
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Target: 5'- aGCAGGCGcCGCacaugCCuUGggGGUCu -3' miRNA: 3'- -CGUCUGCuGCGgaaa-GG-GCuuCCAGu -5' |
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| 30910 | 3' | -53.7 | NC_006552.1 | + | 24733 | 0.67 | 0.808106 |
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Target: 5'- cCAGcCGGCGCCgggCCUGcucGGUCAa -3' miRNA: 3'- cGUCuGCUGCGGaaaGGGCuu-CCAGU- -5' |
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| 30910 | 3' | -53.7 | NC_006552.1 | + | 62405 | 0.67 | 0.798594 |
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Target: 5'- cGCGGaACGcUGCCaugUCCCGAucguGGUCc -3' miRNA: 3'- -CGUC-UGCuGCGGaa-AGGGCUu---CCAGu -5' |
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| 30910 | 3' | -53.7 | NC_006552.1 | + | 20285 | 0.67 | 0.78891 |
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Target: 5'- gGUAGACcuGCGCCagcgUCUCGAAGGcCGc -3' miRNA: 3'- -CGUCUGc-UGCGGaa--AGGGCUUCCaGU- -5' |
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| 30910 | 3' | -53.7 | NC_006552.1 | + | 51647 | 0.67 | 0.78891 |
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Target: 5'- aCGGAuCGcACGCCUgcUgUCGAAGGUCGa -3' miRNA: 3'- cGUCU-GC-UGCGGAa-AgGGCUUCCAGU- -5' |
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| 30910 | 3' | -53.7 | NC_006552.1 | + | 45057 | 0.68 | 0.758933 |
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Target: 5'- uGCuGGCG-UGCCUgcgUUCCGguGGUCGg -3' miRNA: 3'- -CGuCUGCuGCGGAa--AGGGCuuCCAGU- -5' |
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| 30910 | 3' | -53.7 | NC_006552.1 | + | 6924 | 0.69 | 0.706557 |
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Target: 5'- aUAGGCcACGCUguacgUCuuGAAGGUCAu -3' miRNA: 3'- cGUCUGcUGCGGaa---AGggCUUCCAGU- -5' |
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| 30910 | 3' | -53.7 | NC_006552.1 | + | 26439 | 0.74 | 0.413217 |
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Target: 5'- aGC-GACGGCGCCgacUCCUGggGGcCGc -3' miRNA: 3'- -CGuCUGCUGCGGaa-AGGGCuuCCaGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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