Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30912 | 5' | -56.7 | NC_006552.1 | + | 8249 | 0.66 | 0.638925 |
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Target: 5'- cGGGCcaugguguccagGCUACGGUcgGuGGCGgcGCGGa -3' miRNA: 3'- uCCCG------------CGGUGCCAa-CuUCGCuuCGUC- -5' |
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| 30912 | 5' | -56.7 | NC_006552.1 | + | 64511 | 0.67 | 0.617199 |
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Target: 5'- cGGGGUGCUuCGGaUGAAGCaGAaaccaGGCAu -3' miRNA: 3'- -UCCCGCGGuGCCaACUUCG-CU-----UCGUc -5' |
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| 30912 | 5' | -56.7 | NC_006552.1 | + | 44107 | 0.67 | 0.606351 |
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Target: 5'- -cGGCGCCugGGUUGAuAGUGcuucAGCc- -3' miRNA: 3'- ucCCGCGGugCCAACU-UCGCu---UCGuc -5' |
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| 30912 | 5' | -56.7 | NC_006552.1 | + | 31839 | 0.67 | 0.595525 |
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Target: 5'- cGGGugauGCGCCGCaGGUcGGAGuCGggGCGc -3' miRNA: 3'- -UCC----CGCGGUG-CCAaCUUC-GCuuCGUc -5' |
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| 30912 | 5' | -56.7 | NC_006552.1 | + | 13164 | 0.68 | 0.564328 |
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Target: 5'- cAGGGCGUCGCGGgcGuucuGCGuacgcucgaaugccuGGCGGa -3' miRNA: 3'- -UCCCGCGGUGCCaaCuu--CGCu--------------UCGUC- -5' |
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| 30912 | 5' | -56.7 | NC_006552.1 | + | 33260 | 0.68 | 0.510656 |
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Target: 5'- cGGuGGCGCCACc-UUGucgccgauGGCGAGGCAGu -3' miRNA: 3'- -UC-CCGCGGUGccAACu-------UCGCUUCGUC- -5' |
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| 30912 | 5' | -56.7 | NC_006552.1 | + | 56858 | 0.7 | 0.440778 |
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Target: 5'- cGGcaGCGCCGCGGc-GAgcGGCGAAcGCAGg -3' miRNA: 3'- uCC--CGCGGUGCCaaCU--UCGCUU-CGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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