Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30917 | 5' | -59 | NC_006552.1 | + | 32695 | 0.66 | 0.525183 |
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Target: 5'- gUGUCGUCGccGGcCGGCGcgGGGCC-GGUg -3' miRNA: 3'- -ACGGUAGCucCC-GCCGU--UCCGGuUCA- -5' |
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| 30917 | 5' | -59 | NC_006552.1 | + | 60341 | 0.67 | 0.47452 |
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Target: 5'- aUGCCGgacaUUGAGuGGcCGGCGaaGGGCCAGu- -3' miRNA: 3'- -ACGGU----AGCUC-CC-GCCGU--UCCGGUUca -5' |
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| 30917 | 5' | -59 | NC_006552.1 | + | 26118 | 0.67 | 0.454944 |
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Target: 5'- cGCuCAUCGugcaGGUGGU-AGGCCAGGg -3' miRNA: 3'- aCG-GUAGCuc--CCGCCGuUCCGGUUCa -5' |
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| 30917 | 5' | -59 | NC_006552.1 | + | 2627 | 0.67 | 0.44532 |
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Target: 5'- aGCCGauUCGAugGGGCuGGCGgcccuucuGGGCUAGGg -3' miRNA: 3'- aCGGU--AGCU--CCCG-CCGU--------UCCGGUUCa -5' |
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| 30917 | 5' | -59 | NC_006552.1 | + | 32080 | 0.67 | 0.435812 |
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Target: 5'- aGCCcgGUCGGGGGuUGGCAccAGGCgCAgcgaAGUg -3' miRNA: 3'- aCGG--UAGCUCCC-GCCGU--UCCG-GU----UCA- -5' |
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| 30917 | 5' | -59 | NC_006552.1 | + | 33385 | 0.68 | 0.417153 |
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Target: 5'- gGCCcuUCGuAGGGCGGCGGcgcuuagacuGcGCCGAGg -3' miRNA: 3'- aCGGu-AGC-UCCCGCCGUU----------C-CGGUUCa -5' |
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| 30917 | 5' | -59 | NC_006552.1 | + | 31261 | 0.72 | 0.217274 |
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Target: 5'- cGCCGUUGGGGGUgaucGGCA-GGCC-GGUa -3' miRNA: 3'- aCGGUAGCUCCCG----CCGUuCCGGuUCA- -5' |
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| 30917 | 5' | -59 | NC_006552.1 | + | 3731 | 0.74 | 0.171137 |
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Target: 5'- gUGCUGUCGAGGGUguucgcgggGGCGAcaucaauGGCCGAGg -3' miRNA: 3'- -ACGGUAGCUCCCG---------CCGUU-------CCGGUUCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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