Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30919 | 5' | -56.7 | NC_006552.1 | + | 58413 | 0.67 | 0.6554 |
Target: 5'- gGGCAGcAACCGCGagUGcCA-GGCCUCg -3' miRNA: 3'- -CCGUC-UUGGCGUa-GCaGUaCCGGGGg -5' |
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30919 | 5' | -56.7 | NC_006552.1 | + | 58139 | 0.67 | 0.6554 |
Target: 5'- cGCAGccGACUGCAUCGaC-UGGUcguaCCCCa -3' miRNA: 3'- cCGUC--UUGGCGUAGCaGuACCG----GGGG- -5' |
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30919 | 5' | -56.7 | NC_006552.1 | + | 21053 | 0.66 | 0.676727 |
Target: 5'- -cCAGGucgGCCaGCAggUCGUCGauauucaGGCCCCCa -3' miRNA: 3'- ccGUCU---UGG-CGU--AGCAGUa------CCGGGGG- -5' |
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30919 | 5' | -56.7 | NC_006552.1 | + | 53989 | 0.66 | 0.676727 |
Target: 5'- aGGCAGGccaGgCGCGUgGcCcUGGCCCUg -3' miRNA: 3'- -CCGUCU---UgGCGUAgCaGuACCGGGGg -5' |
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30919 | 5' | -56.7 | NC_006552.1 | + | 44798 | 0.66 | 0.708393 |
Target: 5'- aGCGGcAACCGUgaAUCGUCc--GCCCUCc -3' miRNA: 3'- cCGUC-UUGGCG--UAGCAGuacCGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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