Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30919 | 5' | -56.7 | NC_006552.1 | + | 22272 | 0.68 | 0.580591 |
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Target: 5'- cGCGGAgccggucgcucGCCGCAUCGcUgAUGGUUgCCu -3' miRNA: 3'- cCGUCU-----------UGGCGUAGC-AgUACCGGgGG- -5' |
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| 30919 | 5' | -56.7 | NC_006552.1 | + | 21053 | 0.66 | 0.676727 |
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Target: 5'- -cCAGGucgGCCaGCAggUCGUCGauauucaGGCCCCCa -3' miRNA: 3'- ccGUCU---UGG-CGU--AGCAGUa------CCGGGGG- -5' |
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| 30919 | 5' | -56.7 | NC_006552.1 | + | 16363 | 0.67 | 0.644701 |
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Target: 5'- uGCAGAACCGCcgCc----GGCCCgCCc -3' miRNA: 3'- cCGUCUUGGCGuaGcaguaCCGGG-GG- -5' |
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| 30919 | 5' | -56.7 | NC_006552.1 | + | 11262 | 0.69 | 0.517907 |
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Target: 5'- cGCGG-AUCGCGUCGgccaaUAUGGCCCaugCCg -3' miRNA: 3'- cCGUCuUGGCGUAGCa----GUACCGGG---GG- -5' |
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| 30919 | 5' | -56.7 | NC_006552.1 | + | 5509 | 0.67 | 0.644701 |
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Target: 5'- gGGUGGAGCCGUaaccgAUgGUCGgaaUGuCCCCCu -3' miRNA: 3'- -CCGUCUUGGCG-----UAgCAGU---ACcGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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