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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30921 | 5' | -60 | NC_006552.1 | + | 65081 | 0.66 | 0.521249 |
Target: 5'- gAACCUgGacGCCGCCGgCGCCGGcaaacgaagcGGGAGc -3' miRNA: 3'- -UUGGAgC--CGGCGGCaGUGGCU----------CCUUC- -5' |
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30921 | 5' | -60 | NC_006552.1 | + | 63482 | 0.66 | 0.510142 |
Target: 5'- cGCUgcCGGCCGCCGgCGCCGGagcaaccGGAGa -3' miRNA: 3'- uUGGa-GCCGGCGGCaGUGGCU-------CCUUc -5' |
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30921 | 5' | -60 | NC_006552.1 | + | 63071 | 0.67 | 0.471614 |
Target: 5'- gGugCUgGGCauggccaaCGCCGUCGCCGAGa--- -3' miRNA: 3'- -UugGAgCCG--------GCGGCAGUGGCUCcuuc -5' |
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30921 | 5' | -60 | NC_006552.1 | + | 12430 | 0.69 | 0.355287 |
Target: 5'- uGCUUCGGCCGCC-UCGCgGGugcggaacuGGAAGc -3' miRNA: 3'- uUGGAGCCGGCGGcAGUGgCU---------CCUUC- -5' |
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30921 | 5' | -60 | NC_006552.1 | + | 53474 | 0.75 | 0.146663 |
Target: 5'- cGACCUUGGCCGaggcauacaagcaCuUCACCGGGGAGGa -3' miRNA: 3'- -UUGGAGCCGGCg------------GcAGUGGCUCCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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