Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30922 | 5' | -54.1 | NC_006552.1 | + | 22076 | 0.85 | 0.083752 |
Target: 5'- cGCGCGGCAUCCAGCACcaggguGUAC-GCAUGa -3' miRNA: 3'- -CGCGCUGUAGGUCGUG------CAUGuCGUGC- -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 5619 | 0.8 | 0.172582 |
Target: 5'- aGCGCGGCAaCCAGCACGcgguUACGGCuguuCGa -3' miRNA: 3'- -CGCGCUGUaGGUCGUGC----AUGUCGu---GC- -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 52964 | 0.76 | 0.305329 |
Target: 5'- gGUGCGGCAUCCGgguGCGCGUucauCgAGCGCGc -3' miRNA: 3'- -CGCGCUGUAGGU---CGUGCAu---G-UCGUGC- -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 50083 | 0.75 | 0.3191 |
Target: 5'- aGCGCGACAaCC-GCACGcgggcggaacgcGCAGCACGg -3' miRNA: 3'- -CGCGCUGUaGGuCGUGCa-----------UGUCGUGC- -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 26880 | 0.75 | 0.339805 |
Target: 5'- aGCGCGGCAUCUucGGCgACGUuguugaugccggccaGCGGCACc -3' miRNA: 3'- -CGCGCUGUAGG--UCG-UGCA---------------UGUCGUGc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 22371 | 0.74 | 0.396624 |
Target: 5'- uGUGCaGCA-CCAGCAgccCGUACAGCACc -3' miRNA: 3'- -CGCGcUGUaGGUCGU---GCAUGUCGUGc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 33358 | 0.72 | 0.503716 |
Target: 5'- uGCGUGACAgaagggCCAGCACGggcCGGCccuucguaggGCGg -3' miRNA: 3'- -CGCGCUGUa-----GGUCGUGCau-GUCG----------UGC- -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 14119 | 0.71 | 0.556415 |
Target: 5'- cGCGCG-CAUCCGGCGcCGUcaugugAguGCGCu -3' miRNA: 3'- -CGCGCuGUAGGUCGU-GCA------UguCGUGc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 49184 | 0.71 | 0.556415 |
Target: 5'- uGCgGCGGCcugGUCCGGCugGU-CAGCAa- -3' miRNA: 3'- -CG-CGCUG---UAGGUCGugCAuGUCGUgc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 738 | 0.71 | 0.545728 |
Target: 5'- cCGCGACGUgCAGCGCucccCGGCGCu -3' miRNA: 3'- cGCGCUGUAgGUCGUGcau-GUCGUGc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 352 | 0.71 | 0.535109 |
Target: 5'- cCGCGACAg-CGGCACGauauacgGCGGCACc -3' miRNA: 3'- cGCGCUGUagGUCGUGCa------UGUCGUGc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 21862 | 0.7 | 0.588802 |
Target: 5'- cGCGCGcCgccagaugGUCCAGCAC---CAGCGCGg -3' miRNA: 3'- -CGCGCuG--------UAGGUCGUGcauGUCGUGC- -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 20888 | 0.7 | 0.62151 |
Target: 5'- aGCGCuuccAUGUCCGGCAUGgcgaucUGCGGCGCa -3' miRNA: 3'- -CGCGc---UGUAGGUCGUGC------AUGUCGUGc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 32152 | 0.7 | 0.62151 |
Target: 5'- uGCGCGcCAuUCCGGCagcuccACGUcCAGCACc -3' miRNA: 3'- -CGCGCuGU-AGGUCG------UGCAuGUCGUGc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 1709 | 0.69 | 0.643376 |
Target: 5'- cGCuGCGAUAcgUCAGCAC--GCAGCAUGg -3' miRNA: 3'- -CG-CGCUGUa-GGUCGUGcaUGUCGUGC- -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 26393 | 0.69 | 0.654299 |
Target: 5'- -gGUGACuUCCAGCGCGc-CGGCAuCGg -3' miRNA: 3'- cgCGCUGuAGGUCGUGCauGUCGU-GC- -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 13381 | 0.69 | 0.674989 |
Target: 5'- aCGCGGCGuuucgacUCCGGCACGcucaUGCcGCGCu -3' miRNA: 3'- cGCGCUGU-------AGGUCGUGC----AUGuCGUGc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 10790 | 0.68 | 0.708401 |
Target: 5'- gGCGcCGACggcauugcGUCCGGCGaacgaGUACuGCACa -3' miRNA: 3'- -CGC-GCUG--------UAGGUCGUg----CAUGuCGUGc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 39274 | 0.68 | 0.740064 |
Target: 5'- -gGUGACAgCCAGCGCugcCGGCGCa -3' miRNA: 3'- cgCGCUGUaGGUCGUGcauGUCGUGc -5' |
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30922 | 5' | -54.1 | NC_006552.1 | + | 62272 | 0.68 | 0.726444 |
Target: 5'- uGUGCGGCAUCgCAGUacucgcuccgucccGCGgacaccguCGGCGCGa -3' miRNA: 3'- -CGCGCUGUAG-GUCG--------------UGCau------GUCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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