Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30924 | 5' | -54.3 | NC_006552.1 | + | 30868 | 0.65 | 0.820056 |
Target: 5'- gGCGCCGUagcgcacaccguaGUCGAUA--GCCCACUg- -3' miRNA: 3'- -CGCGGUAg------------UAGCUAUacCGGGUGGag -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 36124 | 0.66 | 0.814481 |
Target: 5'- cGCGCCAguguUCuguUCGGUGUuGUCUGCCUg -3' miRNA: 3'- -CGCGGU----AGu--AGCUAUAcCGGGUGGAg -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 12902 | 0.66 | 0.805037 |
Target: 5'- uGCGCCuugauGUCGUCGucgGUGGUguUCGCCa- -3' miRNA: 3'- -CGCGG-----UAGUAGCua-UACCG--GGUGGag -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 25907 | 0.66 | 0.785617 |
Target: 5'- -aGCCAUCcggUGAUGcguUGGUgccuaCCACCUCg -3' miRNA: 3'- cgCGGUAGua-GCUAU---ACCG-----GGUGGAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 18900 | 0.67 | 0.744953 |
Target: 5'- uGCuGCUcggCAUCGAUGuUGGCCUggGCCUUc -3' miRNA: 3'- -CG-CGGua-GUAGCUAU-ACCGGG--UGGAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 55200 | 0.67 | 0.74391 |
Target: 5'- -aGCUggguaacucccagGUCAUCGAgaaccugcUGGCCgACCUCa -3' miRNA: 3'- cgCGG-------------UAGUAGCUau------ACCGGgUGGAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 2063 | 0.67 | 0.734473 |
Target: 5'- cGCGCCggCGUCGugcgcGGCCUGCUgaUCg -3' miRNA: 3'- -CGCGGuaGUAGCuaua-CCGGGUGG--AG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 4949 | 0.67 | 0.734473 |
Target: 5'- uGCGCgGUCAgcUCGGccagcuUGGCCUcaGCCUUg -3' miRNA: 3'- -CGCGgUAGU--AGCUau----ACCGGG--UGGAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 8138 | 0.67 | 0.72389 |
Target: 5'- uGCGCCAggUgGUCGcgcaGUAUcgcGGCCC-CCUCc -3' miRNA: 3'- -CGCGGU--AgUAGC----UAUA---CCGGGuGGAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 43244 | 0.67 | 0.72389 |
Target: 5'- aGCGCUuggaAUCGAUGcUGGCUCACgCUg -3' miRNA: 3'- -CGCGGuag-UAGCUAU-ACCGGGUG-GAg -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 13941 | 0.67 | 0.72389 |
Target: 5'- aGCGCCcgCGUCGc---GGgCCACgUCg -3' miRNA: 3'- -CGCGGuaGUAGCuauaCCgGGUGgAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 33991 | 0.68 | 0.690553 |
Target: 5'- aCGCCGUgGUCGGUGuacuccaagccauUGGU-CGCCUCg -3' miRNA: 3'- cGCGGUAgUAGCUAU-------------ACCGgGUGGAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 21060 | 0.68 | 0.680759 |
Target: 5'- -gGCCAgcaggUCGUCGAUAUucaGGCCC-CCa- -3' miRNA: 3'- cgCGGU-----AGUAGCUAUA---CCGGGuGGag -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 52280 | 0.69 | 0.647885 |
Target: 5'- gGCGUgAUCGUCGAaaaGGCCC-CCg- -3' miRNA: 3'- -CGCGgUAGUAGCUauaCCGGGuGGag -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 26116 | 0.69 | 0.647885 |
Target: 5'- aGCGCuCAUCGUgcaGGUGguaGGCCaggGCCUCg -3' miRNA: 3'- -CGCG-GUAGUAg--CUAUa--CCGGg--UGGAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 48604 | 0.69 | 0.625879 |
Target: 5'- cGUGaCCGUCGUCGAgaacagaGGCggccagauCCGCCUCg -3' miRNA: 3'- -CGC-GGUAGUAGCUaua----CCG--------GGUGGAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 28252 | 0.7 | 0.560285 |
Target: 5'- uGCGCgGaUCGcCGAacaggucuuUGUGcGCCCACCUCg -3' miRNA: 3'- -CGCGgU-AGUaGCU---------AUAC-CGGGUGGAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 7341 | 0.7 | 0.549507 |
Target: 5'- uGCGCCAUCuGUCGugcu-GCCCGCCcCa -3' miRNA: 3'- -CGCGGUAG-UAGCuauacCGGGUGGaG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 14130 | 0.71 | 0.528153 |
Target: 5'- gGCGCCGUCAUgUGA-GUGcGCUCGCuCUCg -3' miRNA: 3'- -CGCGGUAGUA-GCUaUAC-CGGGUG-GAG- -5' |
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30924 | 5' | -54.3 | NC_006552.1 | + | 26570 | 0.72 | 0.456207 |
Target: 5'- cGCGCCAUCGUaGGUGaagauGCCCGCCg- -3' miRNA: 3'- -CGCGGUAGUAgCUAUac---CGGGUGGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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