Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30925 | 3' | -50 | NC_006552.1 | + | 12909 | 0.66 | 0.969024 |
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Target: 5'- uGAUGUcgUCgucGGUGGUGUUCGccacaagcuCGGCCa -3' miRNA: 3'- gCUACA--AGa--CCACUGCAAGUu--------GCCGG- -5' |
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| 30925 | 3' | -50 | NC_006552.1 | + | 14449 | 0.66 | 0.961995 |
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Target: 5'- uGGUGgaaCUGG-GAaaUUCGugGGCCa -3' miRNA: 3'- gCUACaa-GACCaCUgcAAGUugCCGG- -5' |
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| 30925 | 3' | -50 | NC_006552.1 | + | 28009 | 0.67 | 0.94478 |
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Target: 5'- uGAUGUUCUGc-GGCGaguaCGGCGGCg -3' miRNA: 3'- gCUACAAGACcaCUGCaa--GUUGCCGg -5' |
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| 30925 | 3' | -50 | NC_006552.1 | + | 15632 | 0.67 | 0.942817 |
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Target: 5'- gCGgcGUUCUGGUGGCGcugcUCAuccugcugguccuCGGCg -3' miRNA: 3'- -GCuaCAAGACCACUGCa---AGUu------------GCCGg -5' |
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| 30925 | 3' | -50 | NC_006552.1 | + | 11340 | 0.68 | 0.928959 |
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Target: 5'- uCGAUGgcuucCUcGGUGACG----GCGGCCg -3' miRNA: 3'- -GCUACaa---GA-CCACUGCaaguUGCCGG- -5' |
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| 30925 | 3' | -50 | NC_006552.1 | + | 33529 | 0.69 | 0.903899 |
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Target: 5'- --cUGgaaCUGGUGcgcagGCGcgCAGCGGCCg -3' miRNA: 3'- gcuACaa-GACCAC-----UGCaaGUUGCCGG- -5' |
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| 30925 | 3' | -50 | NC_006552.1 | + | 32109 | 0.7 | 0.858097 |
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Target: 5'- gCGAagUGUcgccCUGGaUGGCGUaCAGCGGCUu -3' miRNA: 3'- -GCU--ACAa---GACC-ACUGCAaGUUGCCGG- -5' |
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| 30925 | 3' | -50 | NC_006552.1 | + | 20406 | 0.76 | 0.555243 |
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Target: 5'- -cAUGUUCUGGUGcAUGUUCu-CGGUCa -3' miRNA: 3'- gcUACAAGACCAC-UGCAAGuuGCCGG- -5' |
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| 30925 | 3' | -50 | NC_006552.1 | + | 3741 | 0.76 | 0.533519 |
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Target: 5'- gGGUGUUCgcgGG-GGCGacaUCAAUGGCCg -3' miRNA: 3'- gCUACAAGa--CCaCUGCa--AGUUGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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