Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30925 | 5' | -55.4 | NC_006552.1 | + | 50480 | 0.66 | 0.725709 |
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Target: 5'- gGGGCGgACUGaUUaCCGCGGAACa-- -3' miRNA: 3'- aCCUGCgUGAC-AAcGGCGUCUUGgag -5' |
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| 30925 | 5' | -55.4 | NC_006552.1 | + | 61039 | 0.66 | 0.708626 |
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Target: 5'- -aGGCGCGCUGgcggccgagcauccaUaUGCCGCGuuGGGCCUCc -3' miRNA: 3'- acCUGCGUGAC---------------A-ACGGCGU--CUUGGAG- -5' |
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| 30925 | 5' | -55.4 | NC_006552.1 | + | 4637 | 0.67 | 0.681567 |
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Target: 5'- cGGGCGCgacacgaAUUGUagGCCGCgcucAGggUCUCg -3' miRNA: 3'- aCCUGCG-------UGACAa-CGGCG----UCuuGGAG- -5' |
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| 30925 | 5' | -55.4 | NC_006552.1 | + | 14978 | 0.68 | 0.61684 |
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Target: 5'- cGGAugacccagcuUGCGCUGUUGUCGguGGcgguagGCUUCg -3' miRNA: 3'- aCCU----------GCGUGACAACGGCguCU------UGGAG- -5' |
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| 30925 | 5' | -55.4 | NC_006552.1 | + | 13096 | 0.68 | 0.594903 |
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Target: 5'- cGGugGaagaaGCUGUaGCCGCcuGACUUCa -3' miRNA: 3'- aCCugCg----UGACAaCGGCGucUUGGAG- -5' |
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| 30925 | 5' | -55.4 | NC_006552.1 | + | 18158 | 0.68 | 0.594903 |
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Target: 5'- cGGGCGgAUUGUugUGCCGgAGAAgCUg -3' miRNA: 3'- aCCUGCgUGACA--ACGGCgUCUUgGAg -5' |
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| 30925 | 5' | -55.4 | NC_006552.1 | + | 16091 | 0.68 | 0.594903 |
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Target: 5'- cGGAgCGCuuUGggGCCGCcGGGACCg- -3' miRNA: 3'- aCCU-GCGugACaaCGGCG-UCUUGGag -5' |
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| 30925 | 5' | -55.4 | NC_006552.1 | + | 61661 | 0.69 | 0.540754 |
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Target: 5'- cGGACGUccaacCUGgccGCCGCAcGGACCUg -3' miRNA: 3'- aCCUGCGu----GACaa-CGGCGU-CUUGGAg -5' |
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| 30925 | 5' | -55.4 | NC_006552.1 | + | 64282 | 0.69 | 0.540754 |
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Target: 5'- -cGGCGCGCUGUucugucaccacUGCgGCGGAcGCCUg -3' miRNA: 3'- acCUGCGUGACA-----------ACGgCGUCU-UGGAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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