Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30930 | 3' | -50.6 | NC_006552.1 | + | 35359 | 0.8 | 0.307209 |
Target: 5'- cCGGAGGCUugCcGCCCGGGAuauccgagagacuGGCCGGc -3' miRNA: 3'- -GCUUUUGAugGaCGGGUUCU-------------UCGGCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 61623 | 0.79 | 0.348626 |
Target: 5'- aCGgcAACUGCCugaucuggaUGCCCGAGGccgccGGCCGGa -3' miRNA: 3'- -GCuuUUGAUGG---------ACGGGUUCU-----UCGGCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 11722 | 0.74 | 0.589376 |
Target: 5'- cCGGAAGCacgaugGCCuUGCCgGuGAGGCCGGa -3' miRNA: 3'- -GCUUUUGa-----UGG-ACGGgUuCUUCGGCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 2956 | 0.71 | 0.722676 |
Target: 5'- cCGGuuguGCUGCC-GCCCGAGuuGGcCCGGg -3' miRNA: 3'- -GCUuu--UGAUGGaCGGGUUCu-UC-GGCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 17910 | 0.7 | 0.785532 |
Target: 5'- gCGAAcGCUAUCgGCCCGAG-AGCuauCGGg -3' miRNA: 3'- -GCUUuUGAUGGaCGGGUUCuUCG---GCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 20672 | 0.7 | 0.795499 |
Target: 5'- gCGAAcAGC-GCCUGCUCGauGGgcGCCGGc -3' miRNA: 3'- -GCUU-UUGaUGGACGGGU--UCuuCGGCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 63985 | 0.69 | 0.851215 |
Target: 5'- ---cAACUACCUGgccgCCAAGGgccuGGCCGGc -3' miRNA: 3'- gcuuUUGAUGGACg---GGUUCU----UCGGCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 37167 | 0.69 | 0.859725 |
Target: 5'- uCGAGAACUGaa-GCCUgcGAAGCCuGGg -3' miRNA: 3'- -GCUUUUGAUggaCGGGuuCUUCGG-CC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 37029 | 0.69 | 0.842468 |
Target: 5'- cCGGAcgcGCUGCCaccgcuagcGUCCuGAGAGGCCGGa -3' miRNA: 3'- -GCUUu--UGAUGGa--------CGGG-UUCUUCGGCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 15452 | 0.69 | 0.833493 |
Target: 5'- aCGGAuuccGCUGCCgGCgCCAGcGAuacAGCCGGu -3' miRNA: 3'- -GCUUu---UGAUGGaCG-GGUU-CU---UCGGCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 6299 | 0.68 | 0.867988 |
Target: 5'- gGAuaguGGCUACgCUGgCCGgcuGGAAGCCGa -3' miRNA: 3'- gCUu---UUGAUG-GACgGGU---UCUUCGGCc -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 11137 | 0.68 | 0.875997 |
Target: 5'- aCGGucguACUGCUUGCCgGAcaccuccauGAAGUCGGa -3' miRNA: 3'- -GCUuu--UGAUGGACGGgUU---------CUUCGGCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 12845 | 0.68 | 0.891227 |
Target: 5'- ----uGCUGCgCUGCCCGGcGGGCCuGGa -3' miRNA: 3'- gcuuuUGAUG-GACGGGUUcUUCGG-CC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 42267 | 0.67 | 0.918388 |
Target: 5'- uGGcuGCUGCCUGCCCcuGAGcgugaucaggcGCCaGGc -3' miRNA: 3'- gCUuuUGAUGGACGGGuuCUU-----------CGG-CC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 13247 | 0.67 | 0.92447 |
Target: 5'- uGAGGGCUACaUGCUCAucGAGcGUCGGg -3' miRNA: 3'- gCUUUUGAUGgACGGGUu-CUU-CGGCC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 55245 | 0.66 | 0.941002 |
Target: 5'- aCGAAAGCUACaacgCCAAGAacaaGGCCuGGg -3' miRNA: 3'- -GCUUUUGAUGgacgGGUUCU----UCGG-CC- -5' |
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30930 | 3' | -50.6 | NC_006552.1 | + | 23345 | 0.66 | 0.945945 |
Target: 5'- cCGGcuGCU-CCUGCCgGucgucGAAGUCGGu -3' miRNA: 3'- -GCUuuUGAuGGACGGgUu----CUUCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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