Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30931 | 5' | -54.1 | NC_006552.1 | + | 26270 | 0.66 | 0.841212 |
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Target: 5'- cGUCG-GCAGgaugaCCGUGGCCGCGcccaGCgGGu -3' miRNA: 3'- -CAGCuUGUC-----GGCAUUGGCGCa---CGaCC- -5' |
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| 30931 | 5' | -54.1 | NC_006552.1 | + | 15259 | 0.66 | 0.832438 |
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Target: 5'- cGUCGu-CuGCCGU--CCGC-UGCUGGu -3' miRNA: 3'- -CAGCuuGuCGGCAuuGGCGcACGACC- -5' |
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| 30931 | 5' | -54.1 | NC_006552.1 | + | 11956 | 0.66 | 0.832438 |
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Target: 5'- gGUCGGAgcGCa-UGGCgGUGUGCUGGg -3' miRNA: 3'- -CAGCUUguCGgcAUUGgCGCACGACC- -5' |
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| 30931 | 5' | -54.1 | NC_006552.1 | + | 41016 | 0.66 | 0.823458 |
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Target: 5'- -gCGAACAGCaGUucauCCGCGUugGCUGcGg -3' miRNA: 3'- caGCUUGUCGgCAuu--GGCGCA--CGAC-C- -5' |
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| 30931 | 5' | -54.1 | NC_006552.1 | + | 35870 | 0.68 | 0.724481 |
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Target: 5'- cGUCGGggccucGCcuGCCGUGGCgCGCGcUGCUGc -3' miRNA: 3'- -CAGCU------UGu-CGGCAUUG-GCGC-ACGACc -5' |
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| 30931 | 5' | -54.1 | NC_006552.1 | + | 23527 | 0.68 | 0.724481 |
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Target: 5'- -aCGuAGCAGCCGaauaUAACCGUGUcGCacUGGa -3' miRNA: 3'- caGC-UUGUCGGC----AUUGGCGCA-CG--ACC- -5' |
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| 30931 | 5' | -54.1 | NC_006552.1 | + | 29444 | 0.68 | 0.691445 |
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Target: 5'- uUCGAAUugaAGCCGUAgGCCGaaacgaaCGUGCUGu -3' miRNA: 3'- cAGCUUG---UCGGCAU-UGGC-------GCACGACc -5' |
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| 30931 | 5' | -54.1 | NC_006552.1 | + | 50162 | 0.69 | 0.63825 |
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Target: 5'- --gGAGCAGgCGU-ACUGCGcGCUGGu -3' miRNA: 3'- cagCUUGUCgGCAuUGGCGCaCGACC- -5' |
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| 30931 | 5' | -54.1 | NC_006552.1 | + | 7534 | 0.72 | 0.499166 |
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Target: 5'- aGUCGGcCAGCCGU-ACCGCG-GCg-- -3' miRNA: 3'- -CAGCUuGUCGGCAuUGGCGCaCGacc -5' |
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| 30931 | 5' | -54.1 | NC_006552.1 | + | 38154 | 0.72 | 0.488942 |
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Target: 5'- cGUUGAGCugcuGGCCGcggUAGCUGUugGUGCUGGg -3' miRNA: 3'- -CAGCUUG----UCGGC---AUUGGCG--CACGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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