Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30933 | 3' | -62.8 | NC_006552.1 | + | 13852 | 0.66 | 0.342474 |
Target: 5'- aGGCGCcGGUacugGGCCaGGUaaucGGCCcGCa -3' miRNA: 3'- aUCGCGaCCGa---CCGGaCCA----CCGGuCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 60281 | 0.66 | 0.334596 |
Target: 5'- -cGCGCcacccGGCUGGcCCUGGgcaucGGCCccugGGUg -3' miRNA: 3'- auCGCGa----CCGACC-GGACCa----CCGG----UCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 59609 | 0.66 | 0.326852 |
Target: 5'- aAGCGCaGGCc-GCCUGGcagaacucgGGCCGGg -3' miRNA: 3'- aUCGCGaCCGacCGGACCa--------CCGGUCg -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 14305 | 0.66 | 0.311764 |
Target: 5'- cGGCGCgGGCcugGGCCUcGGccUGuaaccucuuuGCCAGCa -3' miRNA: 3'- aUCGCGaCCGa--CCGGA-CC--AC----------CGGUCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 28915 | 0.66 | 0.311024 |
Target: 5'- cGGUGCUcuggaauccggccGGCUcGGCaaGGUGaGCCAGUu -3' miRNA: 3'- aUCGCGA-------------CCGA-CCGgaCCAC-CGGUCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 43187 | 0.67 | 0.304421 |
Target: 5'- aGGUGCUGGUcguaGGCCUgcucGGUcuuguucaucuGGCCGGUu -3' miRNA: 3'- aUCGCGACCGa---CCGGA----CCA-----------CCGGUCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 53998 | 0.67 | 0.297212 |
Target: 5'- aGGCGCgUGGCccUGGcCCUGGccGCgAGCc -3' miRNA: 3'- aUCGCG-ACCG--ACC-GGACCacCGgUCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 57476 | 0.67 | 0.290135 |
Target: 5'- gUGGCGCcGGUacgGGCUUGGaaaugGGCCugGGCc -3' miRNA: 3'- -AUCGCGaCCGa--CCGGACCa----CCGG--UCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 5058 | 0.67 | 0.290135 |
Target: 5'- aAGCGU--GCUGGCCaccaGGCCAGCc -3' miRNA: 3'- aUCGCGacCGACCGGaccaCCGGUCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 3574 | 0.67 | 0.283192 |
Target: 5'- aGGUGCUGGCcGaGCCgccGGc-GCCGGCa -3' miRNA: 3'- aUCGCGACCGaC-CGGa--CCacCGGUCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 64599 | 0.67 | 0.263151 |
Target: 5'- gUGGUaCUGGUcaUGGcCCUGGUGcuCCAGCa -3' miRNA: 3'- -AUCGcGACCG--ACC-GGACCACc-GGUCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 64492 | 0.68 | 0.256732 |
Target: 5'- --aCGCUGGC-GGCCUGGcaGGCgGGg -3' miRNA: 3'- aucGCGACCGaCCGGACCa-CCGgUCg -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 2742 | 0.68 | 0.237645 |
Target: 5'- -cGCGCUuugccccguuucaGGCUGGUUcgagugGGUGGCCgagGGCg -3' miRNA: 3'- auCGCGA-------------CCGACCGGa-----CCACCGG---UCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 19805 | 0.69 | 0.220879 |
Target: 5'- --uCGCUcaGGUUGGCCUgcagGGUGGCCuuguaGGCg -3' miRNA: 3'- aucGCGA--CCGACCGGA----CCACCGG-----UCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 50335 | 0.71 | 0.149554 |
Target: 5'- cGGCGU--GCUGGcCCUGGUGGCUccguuuuacGGCu -3' miRNA: 3'- aUCGCGacCGACC-GGACCACCGG---------UCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 12639 | 0.76 | 0.067827 |
Target: 5'- cGGCGCUGGCuacgcgggucuUGGCCUGuGccuUGGCCuGCu -3' miRNA: 3'- aUCGCGACCG-----------ACCGGAC-C---ACCGGuCG- -5' |
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30933 | 3' | -62.8 | NC_006552.1 | + | 12949 | 0.82 | 0.021198 |
Target: 5'- -cGCGCUGGCcuUGGCCUGGcggugcguucgaGGCCAGCa -3' miRNA: 3'- auCGCGACCG--ACCGGACCa-----------CCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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