Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30934 | 5' | -62.3 | NC_006552.1 | + | 2053 | 0.67 | 0.372584 |
Target: 5'- gGCGCuuGAgCGCgCCg--GCGuCGUGCGCGg -3' miRNA: 3'- -CGCG--CUgGCG-GGacaCGC-GCACGCGU- -5' |
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30934 | 5' | -62.3 | NC_006552.1 | + | 20632 | 0.66 | 0.406612 |
Target: 5'- cGCGCGGgugguuUCGCCaCUGU-CGCGUuCGCGg -3' miRNA: 3'- -CGCGCU------GGCGG-GACAcGCGCAcGCGU- -5' |
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30934 | 5' | -62.3 | NC_006552.1 | + | 22521 | 0.7 | 0.238554 |
Target: 5'- gGUG-GGCCGCCUUGUGaucgGCGaGCGCGg -3' miRNA: 3'- -CGCgCUGGCGGGACACg---CGCaCGCGU- -5' |
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30934 | 5' | -62.3 | NC_006552.1 | + | 33511 | 0.67 | 0.372584 |
Target: 5'- aGCGCGAacagCGCCUUgcuggaacugGUGCGCaggcGCGCAg -3' miRNA: 3'- -CGCGCUg---GCGGGA----------CACGCGca--CGCGU- -5' |
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30934 | 5' | -62.3 | NC_006552.1 | + | 39550 | 0.69 | 0.269394 |
Target: 5'- -gGCGucacCCGCCaguCUGUGCGCGUGacaGCc -3' miRNA: 3'- cgCGCu---GGCGG---GACACGCGCACg--CGu -5' |
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30934 | 5' | -62.3 | NC_006552.1 | + | 51228 | 0.66 | 0.380907 |
Target: 5'- uGCGCGugaaugaaGCCGCCgaUGaGCGCcUGCGCc -3' miRNA: 3'- -CGCGC--------UGGCGGg-ACaCGCGcACGCGu -5' |
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30934 | 5' | -62.3 | NC_006552.1 | + | 52964 | 0.67 | 0.348363 |
Target: 5'- gGUGCGGCa-UCCggGUGCGCGUucaucgagcGCGCAg -3' miRNA: 3'- -CGCGCUGgcGGGa-CACGCGCA---------CGCGU- -5' |
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30934 | 5' | -62.3 | NC_006552.1 | + | 55471 | 0.66 | 0.424343 |
Target: 5'- -gGCGAUCGCCCUGcUGCaccacaGCGaagGgGCGg -3' miRNA: 3'- cgCGCUGGCGGGAC-ACG------CGCa--CgCGU- -5' |
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30934 | 5' | -62.3 | NC_006552.1 | + | 56463 | 0.66 | 0.406612 |
Target: 5'- cGUG-GGCCGCCCUGUcaggcaGgGCG-GCGUc -3' miRNA: 3'- -CGCgCUGGCGGGACA------CgCGCaCGCGu -5' |
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30934 | 5' | -62.3 | NC_006552.1 | + | 64153 | 0.72 | 0.163394 |
Target: 5'- uCGCG-CCGCCCUG-GCGCacGCGCc -3' miRNA: 3'- cGCGCuGGCGGGACaCGCGcaCGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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