Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30937 | 5' | -58.5 | NC_006552.1 | + | 36165 | 0.66 | 0.577761 |
Target: 5'- cAUCgGCGcuGGCCuCGCcaGCaUCGCCGGc -3' miRNA: 3'- -UAGaCGCuuUCGG-GCG--UG-AGCGGCCu -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 53514 | 0.66 | 0.577761 |
Target: 5'- -cCUgGUGGAGGCucaCCGCGCgaUGCCGGAc -3' miRNA: 3'- uaGA-CGCUUUCG---GGCGUGa-GCGGCCU- -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 12379 | 0.66 | 0.55655 |
Target: 5'- uUCUGCaacGGCCUGCGCgUCGgCGGu -3' miRNA: 3'- uAGACGcuuUCGGGCGUG-AGCgGCCu -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 56901 | 0.66 | 0.546028 |
Target: 5'- cGUCgGCGAGgaguGGUaCCGCGCccgCGCUGGAc -3' miRNA: 3'- -UAGaCGCUU----UCG-GGCGUGa--GCGGCCU- -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 36384 | 0.66 | 0.546028 |
Target: 5'- --aUGCGGAuuacUCCGCGuCUCGCCGGc -3' miRNA: 3'- uagACGCUUuc--GGGCGU-GAGCGGCCu -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 26779 | 0.66 | 0.544979 |
Target: 5'- -cCUgGCGcacaGAGGCCCGCAguugcgcCUUGCCGGc -3' miRNA: 3'- uaGA-CGC----UUUCGGGCGU-------GAGCGGCCu -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 48497 | 0.66 | 0.53557 |
Target: 5'- -aCUGCGAgccggccuGAGCCCGCGCgcccaGCauccugaaCGGAg -3' miRNA: 3'- uaGACGCU--------UUCGGGCGUGag---CG--------GCCU- -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 11582 | 0.66 | 0.534528 |
Target: 5'- -aCUGCGccggcccAGGGCCUGCACcaguUCGCCGc- -3' miRNA: 3'- uaGACGC-------UUUCGGGCGUG----AGCGGCcu -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 43995 | 0.67 | 0.525183 |
Target: 5'- uGUCgGCGAccaGGGCCagGCGaacCUCGCCGGu -3' miRNA: 3'- -UAGaCGCU---UUCGGg-CGU---GAGCGGCCu -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 51996 | 0.67 | 0.518988 |
Target: 5'- ---aGCGAGAGCuuGCcgcggcgcgccagcaACUCGCCGa- -3' miRNA: 3'- uagaCGCUUUCGggCG---------------UGAGCGGCcu -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 14135 | 0.67 | 0.514874 |
Target: 5'- cGUCaUGUGAGugcGCUCGCuCUCGcCCGGGa -3' miRNA: 3'- -UAG-ACGCUUu--CGGGCGuGAGC-GGCCU- -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 30471 | 0.7 | 0.316119 |
Target: 5'- -cCUGCGAucGCCUGUgaguagugcguACUCGCCGaGGu -3' miRNA: 3'- uaGACGCUuuCGGGCG-----------UGAGCGGC-CU- -5' |
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30937 | 5' | -58.5 | NC_006552.1 | + | 56645 | 0.74 | 0.18578 |
Target: 5'- -gCUGCgGAAGGCCCGCAgCUaCGCCGaGGu -3' miRNA: 3'- uaGACG-CUUUCGGGCGU-GA-GCGGC-CU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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