Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30938 | 3' | -59.4 | NC_006552.1 | + | 34503 | 0.71 | 0.240984 |
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Target: 5'- gAACUucaCGGGCGGCGGCgGCAGCUcgaucuGGUCc -3' miRNA: 3'- -UUGA---GCCCGCCGUCG-UGUUGG------CCAGa -5' |
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| 30938 | 3' | -59.4 | NC_006552.1 | + | 29294 | 0.71 | 0.266439 |
|
Target: 5'- gGAUUCGcgccccacGGCGGCGGCGCAauguuGCCGGcCg -3' miRNA: 3'- -UUGAGC--------CCGCCGUCGUGU-----UGGCCaGa -5' |
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| 30938 | 3' | -59.4 | NC_006552.1 | + | 21148 | 0.68 | 0.384601 |
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Target: 5'- ---aCGGGCGGCGGCgACGcgauaggcgcauccgGCCGcGUCg -3' miRNA: 3'- uugaGCCCGCCGUCG-UGU---------------UGGC-CAGa -5' |
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| 30938 | 3' | -59.4 | NC_006552.1 | + | 59460 | 0.67 | 0.435381 |
|
Target: 5'- cACUUGGGCGGCugAGCu--GCUGGUg- -3' miRNA: 3'- uUGAGCCCGCCG--UCGuguUGGCCAga -5' |
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| 30938 | 3' | -59.4 | NC_006552.1 | + | 59759 | 0.67 | 0.452508 |
|
Target: 5'- uGCUCGaguacGGCGGCcGCAUcggcuuucugcuACCGGUCUa -3' miRNA: 3'- uUGAGC-----CCGCCGuCGUGu-----------UGGCCAGA- -5' |
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| 30938 | 3' | -59.4 | NC_006552.1 | + | 44598 | 0.67 | 0.453471 |
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Target: 5'- cGCUCGuaGGCGGCAaugaacgccaugcGCGCGcccacCCGGUCa -3' miRNA: 3'- uUGAGC--CCGCCGU-------------CGUGUu----GGCCAGa -5' |
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| 30938 | 3' | -59.4 | NC_006552.1 | + | 15603 | 0.67 | 0.454434 |
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Target: 5'- gAGCaCGGGCGGCuAGCuCAACcCGGcCa -3' miRNA: 3'- -UUGaGCCCGCCG-UCGuGUUG-GCCaGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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