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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30940 | 5' | -55.1 | NC_006552.1 | + | 45360 | 0.69 | 0.594668 |
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Target: 5'- gGCAUGUCGgccgguaucUGGCAGCgcGGcCCGGGCu- -3' miRNA: 3'- -CGUGCAGC---------AUCGUCG--UCaGGCUCGuc -5' |
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| 30940 | 5' | -55.1 | NC_006552.1 | + | 25381 | 0.68 | 0.627339 |
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Target: 5'- cCGCGgCGaaguUAGCAGCGGUCCaguuAGCAGc -3' miRNA: 3'- cGUGCaGC----AUCGUCGUCAGGc---UCGUC- -5' |
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| 30940 | 5' | -55.1 | NC_006552.1 | + | 30878 | 0.68 | 0.653514 |
|
Target: 5'- cGCACaccguaGUCGauagcccacugcugcUGGCAGCGGUC-GGGCGGa -3' miRNA: 3'- -CGUG------CAGC---------------AUCGUCGUCAGgCUCGUC- -5' |
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| 30940 | 5' | -55.1 | NC_006552.1 | + | 58614 | 0.68 | 0.660045 |
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Target: 5'- -gGCGUCgGUGGCcGCg--CCGGGCAGa -3' miRNA: 3'- cgUGCAG-CAUCGuCGucaGGCUCGUC- -5' |
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| 30940 | 5' | -55.1 | NC_006552.1 | + | 18199 | 0.67 | 0.681739 |
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Target: 5'- uCACGUUGUugGGCA--GGUCCGAGCc- -3' miRNA: 3'- cGUGCAGCA--UCGUcgUCAGGCUCGuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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