Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30941 | 5' | -53.9 | NC_006552.1 | + | 40778 | 0.66 | 0.835693 |
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Target: 5'- gGCUGGCGUGauUCGCcucGGCGAucugcaCCAUCu- -3' miRNA: 3'- -CGACUGCAU--AGCG---UCGCUc-----GGUAGca -5' |
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| 30941 | 5' | -53.9 | NC_006552.1 | + | 1911 | 0.66 | 0.826723 |
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Target: 5'- uGCUacGACGUGcUCGaCGGCGGcGUCGUUGUc -3' miRNA: 3'- -CGA--CUGCAU-AGC-GUCGCU-CGGUAGCA- -5' |
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| 30941 | 5' | -53.9 | NC_006552.1 | + | 48465 | 0.67 | 0.782989 |
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Target: 5'- cGCUGGCGUGUCcgguuGCGuugcccuuucccacuGCGAGCCGg--- -3' miRNA: 3'- -CGACUGCAUAG-----CGU---------------CGCUCGGUagca -5' |
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| 30941 | 5' | -53.9 | NC_006552.1 | + | 59361 | 0.67 | 0.779015 |
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Target: 5'- cGCcGAUGgcagcgAUCGC-GCGAGCCAaCGa -3' miRNA: 3'- -CGaCUGCa-----UAGCGuCGCUCGGUaGCa -5' |
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| 30941 | 5' | -53.9 | NC_006552.1 | + | 31056 | 0.67 | 0.758794 |
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Target: 5'- cGCUGGCGgcUUGCc-CGAGCCAcUCa- -3' miRNA: 3'- -CGACUGCauAGCGucGCUCGGU-AGca -5' |
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| 30941 | 5' | -53.9 | NC_006552.1 | + | 10454 | 0.68 | 0.716893 |
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Target: 5'- aGCgaGACGg--CGCAgGCGAGCCAgCGc -3' miRNA: 3'- -CGa-CUGCauaGCGU-CGCUCGGUaGCa -5' |
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| 30941 | 5' | -53.9 | NC_006552.1 | + | 61141 | 0.72 | 0.491301 |
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Target: 5'- aGCaGGCGUuggcagagggagugCGCGGCGAGCCAUgCGa -3' miRNA: 3'- -CGaCUGCAua------------GCGUCGCUCGGUA-GCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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