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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30954 | 5' | -49.9 | NC_006556.1 | + | 14315 | 0.66 | 0.721554 |
Target: 5'- aUAAACAUGuu--GGCCCuauucggGCCGGa -3' miRNA: 3'- gGUUUGUACuuauCCGGGua-----CGGCUa -5' |
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30954 | 5' | -49.9 | NC_006556.1 | + | 16636 | 0.67 | 0.672796 |
Target: 5'- --cGGCAUGu---GuGCCCGUGCCGGg -3' miRNA: 3'- gguUUGUACuuauC-CGGGUACGGCUa -5' |
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30954 | 5' | -49.9 | NC_006556.1 | + | 1638 | 0.68 | 0.573854 |
Target: 5'- aUAAACGcUGGG-AGGUCCAUGCCGc- -3' miRNA: 3'- gGUUUGU-ACUUaUCCGGGUACGGCua -5' |
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30954 | 5' | -49.9 | NC_006556.1 | + | 14829 | 0.71 | 0.40208 |
Target: 5'- aCAAACGcggcUGAGUAGGCUgA-GCCGGUg -3' miRNA: 3'- gGUUUGU----ACUUAUCCGGgUaCGGCUA- -5' |
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30954 | 5' | -49.9 | NC_006556.1 | + | 6834 | 1.08 | 0.001072 |
Target: 5'- cCCAAACAUGAAUAGGCCCAUGCCGAUu -3' miRNA: 3'- -GGUUUGUACUUAUCCGGGUACGGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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