Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30955 | 5' | -51.1 | NC_006556.1 | + | 19778 | 0.66 | 0.679873 |
Target: 5'- aGCACCCUCcuUAUCGuuGaaauacgugGGCGCc -3' miRNA: 3'- -CGUGGGAG--AUAGUggCcuaua----UCGCG- -5' |
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30955 | 5' | -51.1 | NC_006556.1 | + | 843 | 0.67 | 0.624229 |
Target: 5'- -gGCCCaugacCACUGGAaUAUGGCGCu -3' miRNA: 3'- cgUGGGagauaGUGGCCU-AUAUCGCG- -5' |
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30955 | 5' | -51.1 | NC_006556.1 | + | 14994 | 0.67 | 0.587951 |
Target: 5'- gGCGgCCUCUAUagGCCaGAUAccUAGCGg -3' miRNA: 3'- -CGUgGGAGAUAg-UGGcCUAU--AUCGCg -5' |
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30955 | 5' | -51.1 | NC_006556.1 | + | 18228 | 0.74 | 0.251029 |
Target: 5'- aGC-CCCUCUA-CGCCGGAUuu-GCGa -3' miRNA: 3'- -CGuGGGAGAUaGUGGCCUAuauCGCg -5' |
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30955 | 5' | -51.1 | NC_006556.1 | + | 7333 | 1.15 | 0.000276 |
Target: 5'- gGCACCCUCUAUCACCGGAUAUAGCGCg -3' miRNA: 3'- -CGUGGGAGAUAGUGGCCUAUAUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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