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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30956 | 3' | -58.9 | NC_006556.1 | + | 7825 | 1.07 | 0.000158 |
Target: 5'- uUCGGCUGUGGCGGGUGGGACACGUUAu -3' miRNA: 3'- -AGCCGACACCGCCCACCCUGUGCAAU- -5' |
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30956 | 3' | -58.9 | NC_006556.1 | + | 13723 | 0.67 | 0.222038 |
Target: 5'- gUGGCUugagcaggauucgaGUGGCGGGUGGuGGUugGUc- -3' miRNA: 3'- aGCCGA--------------CACCGCCCACC-CUGugCAau -5' |
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30956 | 3' | -58.9 | NC_006556.1 | + | 3627 | 0.66 | 0.243169 |
Target: 5'- gCGGUUGUGGCGGGgcu--CACGa-- -3' miRNA: 3'- aGCCGACACCGCCCacccuGUGCaau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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