Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30956 | 5' | -56.8 | NC_006556.1 | + | 19294 | 0.66 | 0.305288 |
Target: 5'- uGCCAugGCGAuUGCCaaCGCCAucauuaGCGCCAGa -3' miRNA: 3'- -CGGU--CGUU-AUGGcgGCGGU------UGCGGUU- -5' |
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30956 | 5' | -56.8 | NC_006556.1 | + | 13593 | 0.67 | 0.266475 |
Target: 5'- -aCGGCAuuauguUGCCGUCGUaCAACGCCu- -3' miRNA: 3'- cgGUCGUu-----AUGGCGGCG-GUUGCGGuu -5' |
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30956 | 5' | -56.8 | NC_006556.1 | + | 10594 | 0.67 | 0.266475 |
Target: 5'- aGCCGGCA--ACC-CCaGCCAaaGCGCCc- -3' miRNA: 3'- -CGGUCGUuaUGGcGG-CGGU--UGCGGuu -5' |
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30956 | 5' | -56.8 | NC_006556.1 | + | 11584 | 0.68 | 0.247919 |
Target: 5'- gGCCAGCGAUACUaauGUCGUCAcgauaaagacuauggGgGCCAGu -3' miRNA: 3'- -CGGUCGUUAUGG---CGGCGGU---------------UgCGGUU- -5' |
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30956 | 5' | -56.8 | NC_006556.1 | + | 4818 | 0.68 | 0.224643 |
Target: 5'- cCCAGCAcuaaaugcGUACCGauaaCGCCAACgacucaaGCCGAa -3' miRNA: 3'- cGGUCGU--------UAUGGCg---GCGGUUG-------CGGUU- -5' |
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30956 | 5' | -56.8 | NC_006556.1 | + | 19871 | 0.68 | 0.218342 |
Target: 5'- uGCCGGCAcacauaaGUGCCGCgGCUgagGAUuugGCCAAa -3' miRNA: 3'- -CGGUCGU-------UAUGGCGgCGG---UUG---CGGUU- -5' |
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30956 | 5' | -56.8 | NC_006556.1 | + | 7040 | 0.69 | 0.20092 |
Target: 5'- aGCCGGCAAUACggguaaUGCgGCCAGC-CUAGa -3' miRNA: 3'- -CGGUCGUUAUG------GCGgCGGUUGcGGUU- -5' |
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30956 | 5' | -56.8 | NC_006556.1 | + | 15355 | 0.69 | 0.184181 |
Target: 5'- gGCCAGU---AUCGCUauuaugGCCAACGCCGc -3' miRNA: 3'- -CGGUCGuuaUGGCGG------CGGUUGCGGUu -5' |
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30956 | 5' | -56.8 | NC_006556.1 | + | 11786 | 0.72 | 0.121433 |
Target: 5'- cGCCccCAAcUACCGCCccaacaacuaaaGCCAACGCCGAa -3' miRNA: 3'- -CGGucGUU-AUGGCGG------------CGGUUGCGGUU- -5' |
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30956 | 5' | -56.8 | NC_006556.1 | + | 11189 | 0.83 | 0.017858 |
Target: 5'- uGCCAGCAAUAUCGCCucagcccacaugGCgGACGCCAAu -3' miRNA: 3'- -CGGUCGUUAUGGCGG------------CGgUUGCGGUU- -5' |
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30956 | 5' | -56.8 | NC_006556.1 | + | 7787 | 1.08 | 0.000161 |
Target: 5'- cGCCAGCAAUACCGCCGCCAACGCCAAa -3' miRNA: 3'- -CGGUCGUUAUGGCGGCGGUUGCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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