Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30958 | 3' | -55.5 | NC_006556.1 | + | 19214 | 0.66 | 0.449937 |
Target: 5'- -uUCGCCGgaaaGCaccaUAUCCGACAGCa -3' miRNA: 3'- ccGGCGGCg---CGaua-AUAGGCUGUCGg -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 15301 | 0.66 | 0.433335 |
Target: 5'- uGGCCGCgGCGUUGgccauaauagCGAUacuGGCCa -3' miRNA: 3'- -CCGGCGgCGCGAUaauag-----GCUG---UCGG- -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 13170 | 0.66 | 0.42924 |
Target: 5'- gGGCCaCCGUaCUGgugcaUGUgCCGGCAGUCa -3' miRNA: 3'- -CCGGcGGCGcGAUa----AUA-GGCUGUCGG- -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 11613 | 0.67 | 0.389588 |
Target: 5'- aGGCCGCUGaGCcuuagUAUaagcaUGGCGGCCa -3' miRNA: 3'- -CCGGCGGCgCGaua--AUAg----GCUGUCGG- -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 9909 | 0.67 | 0.380057 |
Target: 5'- gGGCgGCUugauGCGCUA--AUUCGGC-GCCa -3' miRNA: 3'- -CCGgCGG----CGCGAUaaUAGGCUGuCGG- -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 4870 | 0.67 | 0.352413 |
Target: 5'- gGGuuGCCGuCcCUAUgg-CgGACGGCCu -3' miRNA: 3'- -CCggCGGC-GcGAUAauaGgCUGUCGG- -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 15365 | 0.68 | 0.343519 |
Target: 5'- aGGCCGCUauggccaguauCGCUAUUAUggccaacgCCG-CGGCCa -3' miRNA: 3'- -CCGGCGGc----------GCGAUAAUA--------GGCuGUCGG- -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 2837 | 0.69 | 0.263567 |
Target: 5'- aGCCGCCGCGUUGaUGUaCCcACAcCCg -3' miRNA: 3'- cCGGCGGCGCGAUaAUA-GGcUGUcGG- -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 4199 | 0.7 | 0.252272 |
Target: 5'- uGGCCaggacgauacugacgGCCGCGgUAgaggGUCCuagGGCGGCCc -3' miRNA: 3'- -CCGG---------------CGGCGCgAUaa--UAGG---CUGUCGG- -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 7280 | 0.71 | 0.205149 |
Target: 5'- aGUgGCCGCGCUA-UAUCCGgugauagagGguGCCg -3' miRNA: 3'- cCGgCGGCGCGAUaAUAGGC---------UguCGG- -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 2412 | 0.79 | 0.05487 |
Target: 5'- aGCCGCCGCGUcaacGUCCGugGGUCu -3' miRNA: 3'- cCGGCGGCGCGauaaUAGGCugUCGG- -5' |
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30958 | 3' | -55.5 | NC_006556.1 | + | 10741 | 1.15 | 9.9e-05 |
Target: 5'- aGGCCGCCGCGCUAUUAUCCGACAGCCa -3' miRNA: 3'- -CCGGCGGCGCGAUAAUAGGCUGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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